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Morphological, immunohistochemical, and genomic analyses of papillary renal neoplasm with reverse polarity
- Source :
- Human pathology. 112
- Publication Year :
- 2021
-
Abstract
- Papillary renal neoplasm with reverse polarity (PRNRP) is a recently proposed entity of renal tumor. It shows a far better prognosis than papillary renal cell carcinoma (PRCC) and frequently has KRAS missense mutation. In this study, we compared 14 cases of PRNRP and 10 cases of PRCC type 1 (PRCC1) and type 2 (PRCC2) from clinical, morphological, immunohistochemical, and molecular biological perspectives. We subjected all PRNRP and PRCC cases to immunohistochemical analysis. Whole-exome sequencing using next-generation sequencing (NGS) was performed for six cases of PRNRP, three cases of PRCC1, and four cases of PRCC2. A search for KRAS gene mutation in the remaining eight cases of PRNRP was performed by polymerase chain reaction (PCR) sequencing. The results showed that all cases of PRNRP were pT1N0M0, none of which followed a course of recurrence or tumor-related death. Immunohistochemical analysis revealed diffuse staining of CK7, EMA, PAX8, and GATA3 but weak or negative staining of CD10, CD15, and AMACR in PRNRP. By NGS and PCR, KRAS missense mutation was detected in 11 of 14 PRNRP cases, although pathogenic KRAS mutation was not observed in PRCC1 and PRCC2. NGS analysis revealed less tumor mutation burden in PRNRP than in PRCC. PRNRP also showed no specific chromosomal copy number abnormalities, including gains of 7 and 17. In conclusion, we propose that PRNRP is a distinct condition from PRCC.
- Subjects :
- 0301 basic medicine
Adult
Male
Pathology
medicine.medical_specialty
Biology
medicine.disease_cause
Pathology and Forensic Medicine
Renal neoplasm
03 medical and health sciences
0302 clinical medicine
KRAS Gene Mutation
medicine
Biomarkers, Tumor
Missense mutation
Humans
Carcinoma, Renal Cell
Aged
Papillary renal cell carcinomas
Middle Aged
Immunohistochemistry
Kidney Neoplasms
030104 developmental biology
030220 oncology & carcinogenesis
Mutation (genetic algorithm)
Mutation
Female
KRAS
PAX8
Subjects
Details
- ISSN :
- 15328392
- Volume :
- 112
- Database :
- OpenAIRE
- Journal :
- Human pathology
- Accession number :
- edsair.doi.dedup.....1cf7c8b97a320c92d2b03bc2edecc8cb