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Oropharyngeal microbiome evaluation highlights Neisseria abundance in active celiac patients
- Source :
- Scientific reports (Nature Publishing Group) 8 (2018). doi:10.1038/s41598-018-29443-1, info:cnr-pdr/source/autori:Iaffaldano L.; Granata I.; Pagliuca C.; Esposito M.V.; Casaburi G.; Salerno G.; Colicchio R.; Piccirillo M.; Ciacci C.; Del Vecchio Blanco G.; Guarracino M.R.; Salvatore P.; Salvatore F.; D'Argenio V.; Sacchetti L./titolo:Oropharyngeal microbiome evaluation highlights Neisseria abundance in active celiac patients/doi:10.1038%2Fs41598-018-29443-1/rivista:Scientific reports (Nature Publishing Group)/anno:2018/pagina_da:/pagina_a:/intervallo_pagine:/volume:8, Scientific Reports, Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
- Publication Year :
- 2018
-
Abstract
- We previously profiled duodenal microbiome in active (a-), gluten-free diet (GFD) celiac disease (CD) patients and controls finding higher levels of the Proteobacterium Neisseria flavescens in a-CD patients than in the other two groups. Here, we investigate the oropharyngeal microbiome in CD patients and controls to evaluate whether this niche share microbial composition with the duodenum. We characterized by 16S rRNA gene sequencing the oropharyngeal microbiome in 14 a-CD, 22 GFD patients and 20 controls. Bacteroidetes, Proteobacteria and Firmicutes differed significantly between the three groups. In particular, Proteobacteria abounded in a-CD and Neisseria species mostly accounted for this abundance (p Neisseria species in a-CD. Microbial functions prediction indicated a greater metabolic potential for degradation of aminoacids, lipids and ketone bodies in a-CD microbiome than in control and GFD microbiomes, in which polysaccharide metabolism predominated. Our results suggest a continuum of a-CD microbial composition from mouth to duodenum. We may speculate that microbiome characterization in the oropharynx, which is a less invasive sampling than the duodenum, could contribute to investigate the role of dysbiosis in CD pathogenesis.
- Subjects :
- 0301 basic medicine
Male
16S
Firmicutes
RNA 16S
lcsh:Medicine
microbiome
Oropharynx
Neisseria flavescens
Biology
Article
Microbiology
Settore MED/12
03 medical and health sciences
RNA, Ribosomal, 16S
medicine
Humans
genetics
Microbiome
human
procedures
lcsh:Science
Ribosomal
Multidisciplinary
biology
isolation and purification
Microbiota
lcsh:R
microbiology
Bacteroidetes
Computational Biology
biology.organism_classification
medicine.disease
RNA 16S, biology
celiac disease
female
male
microflora
Neisseria
oropharynx
physiology
procedures, Celiac Disease
Female
Celiac Disease
030104 developmental biology
medicine.anatomical_structure
Duodenum
RNA
lcsh:Q
Proteobacteria
Dysbiosis
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Scientific reports (Nature Publishing Group) 8 (2018). doi:10.1038/s41598-018-29443-1, info:cnr-pdr/source/autori:Iaffaldano L.; Granata I.; Pagliuca C.; Esposito M.V.; Casaburi G.; Salerno G.; Colicchio R.; Piccirillo M.; Ciacci C.; Del Vecchio Blanco G.; Guarracino M.R.; Salvatore P.; Salvatore F.; D'Argenio V.; Sacchetti L./titolo:Oropharyngeal microbiome evaluation highlights Neisseria abundance in active celiac patients/doi:10.1038%2Fs41598-018-29443-1/rivista:Scientific reports (Nature Publishing Group)/anno:2018/pagina_da:/pagina_a:/intervallo_pagine:/volume:8, Scientific Reports, Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
- Accession number :
- edsair.doi.dedup.....1cda78e81fb0a6bb7dc30b9dc4032b44