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Occurrence of Third-Generation Cephalosporins-Resistant Klebsiella pneumoniae in Fresh Fruits and Vegetables Purchased at Markets in Algeria
- Source :
- Microbial Drug Resistance, Microbial Drug Resistance, Mary Ann Liebert, 2019, pp.1-7. ⟨10.1089/mdr.2019.0249⟩
- Publication Year :
- 2020
- Publisher :
- Mary Ann Liebert Inc, 2020.
-
Abstract
- International audience; This study aimed to characterize third-generation cephalosporin (3GC)-resistant Klebsiella pneumoniae isolated from fresh fruits and vegetables purchased at Bejaia city, Algeria. K. pneumoniae isolates were identified by MALDI-TOF. Susceptibility to antibiotics was tested by the disk diffusion method. Whole genome sequencing (WGS) was carried out to determine sequence type (ST), plasmid incompatibility group (Inc.), and acquired antimicrobial resistance gene presence. A total of 13 3GC-resistant K. pneumoniae strains were isolated. WGS identified blaCTX-M-15 in 11 extended-spectrum-beta-lactamases (ESBL)-K. pneumoniae and blaDHA-1 in 2 AmpC-K. pneumoniae. The aac(6')lb-cr gene was identified in 8 out of 13 isolates. Multilocus sequence typing (MLST) evidenced five different STs, namely ST14, ST45, ST219, ST236, and ST882. MDR K. pneumoniae contaminated fresh fruits and vegetables, often eaten raw and inappropriately washed, may represent an underestimated public health threat. This study highlights that hygiene measures during harvesting and retail process are of utmost importance to limit further ESBL/AmpC spread to the consumer households.
- Subjects :
- Microbiology (medical)
antibiotic resistance
cephalosporine
medicine.drug_class
Klebsiella pneumoniae
cephalosporin
Immunology
Antibiotics
Cephalosporin
résistance aux antibiotiques
veterinary drug
Biology
Microbiology
antibiotique
Third generation cephalosporins
03 medical and health sciences
Antibiotic resistance
antibiotic
medicine
vegetable
Agar diffusion test
microbiologie
bacteria
sécurité des aliments
030304 developmental biology
bactérie
Pharmacology
0303 health sciences
030306 microbiology
fruit
biology.organism_classification
3. Good health
klebsiella pneumoniae
food safety
Algérie
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Algeria
Fruits and vegetables
Multilocus sequence typing
végétaux
Subjects
Details
- ISSN :
- 19318448 and 10766294
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Microbial Drug Resistance
- Accession number :
- edsair.doi.dedup.....1cd5acfd5ca71ef4329c3e2a53785c6d