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Insight into the prebiotic concept: lessons from an exploratory, double blind intervention study with inulin-type fructans in obese women
- Source :
- Gut, Gut, 62(8), 1112-1121, Gut 62 (2013) 8
- Publication Year :
- 2012
- Publisher :
- BMJ Publishing Group, 2012.
-
Abstract
- Objective To highlight the contribution of the gut microbiota to the modulation of host metabolism by dietary inulin-type fructans (ITF prebiotics) in obese women.\ud Methods A double blind, placebo controlled, intervention study was performed with 30 obese women treated with ITF prebiotics (inulin/oligofructose 50/50 mix; n=15) or placebo (maltodextrin; n=15) for\ud 3 months (16 g/day). Blood, faeces and urine sampling, oral glucose tolerance test, homeostasis model assessment and impedancemetry were performed before and after treatment. The gut microbial composition in faeces was analysed by phylogenetic microarray and qPCR analysis of 16S rDNA. Plasma and urine metabolic profiles were analysed by 1H-NMR spectroscopy. Results Treatment with ITF prebiotics, but not the placebo, led to an increase in Bifidobacterium and Faecalibacterium prausnitzii; both bacteria negatively correlated with serum lipopolysaccharide levels. ITF prebiotics also decreased Bacteroides intestinalis, Bacteroides vulgatus and Propionibacterium, an effect associated with a slight decrease in fat mass and with plasma lactate and phosphatidylcholine levels. No clear treatment clustering could be detected for gut microbial analysis or plasma and urine metabolomic profile analyses. However, ITF prebiotics led to subtle changes in the gut microbiota that may importantly impact on several key metabolites implicated in obesity and/or diabetes.\ud Conclusions ITF prebiotics selectively changed the gut microbiota composition in obese women, leading to modest changes in host metabolism, as suggested by the correlation between some bacterial species and metabolic endotoxaemia or metabolomic signatures.
- Subjects :
- human colonic microbiota
medicine.medical_treatment
Physiology
Faecalibacterium prausnitzii
Prebiotic
Gut flora
Body Mass Index
Feces
chemistry.chemical_compound
Microbiologie
Bifidobacterium
Anthropometry
biology
Gastroenterology
Inulin
Middle Aged
Glucagon-like peptide-1
Bacterial Typing Techniques
Metabolome
Female
Adult
Adolescent
Microbiology
high-fat-diet
insulin-resistance
Intestinal Bacteria
Young Adult
Insulin resistance
Double-Blind Method
induced weight-loss
medicine
Humans
oligofructose promotes satiety
Obesity
VLAG
bacteroides-vulgatus
Nutrition
WIMEK
fecal microbiota
Bacteria
gut microbiota
medicine.disease
biology.organism_classification
Prebiotics
glucagon-like peptide-1
chemistry
Metagenome
lipid-metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 14683288 and 00175749
- Volume :
- 62
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Gut
- Accession number :
- edsair.doi.dedup.....1cb3a1178580432c381ecdedfe1d16d8