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Mutation of a raft-targeting signal in the transmembrane region retards transport of influenza virus hemagglutinin through the Golgi
- Source :
- FEBS letters. 586(3)
- Publication Year :
- 2011
-
Abstract
- Inclusion of proteins into membrane-rafts favours interactions required for virus assembly but has also been proposed to facilitate vesicular transport of proteins. The hemagglutinin (HA) of influenza virus contains a raft-targeting sequence in the outer leaflet of its transmembrane region. We report that its mutation enhances co-localization of HA with a cis-Golgi marker and retards Golgi-localized processing, such as acquisition of Endo-H resistant carbohydrates and proteolytic cleavage. In contrast, trimerization of the molecule in the ER and transport to the apical membrane were not affected. The second signal for raft-targeting, S-acylation at cytoplasmic cysteines, did not retard HA transport.
- Subjects :
- Biophysics
Hemagglutinin (influenza)
Golgi Apparatus
Hemagglutinin Glycoproteins, Influenza Virus
Biology
Protein Sorting Signals
medicine.disease_cause
Biochemistry
Exocytosis
Cell Line
symbols.namesake
Dogs
Membrane Microdomains
Palmitoylation
Structural Biology
Golgi
Genetics
medicine
Animals
Hemagglutinin
Molecular Biology
Raft
Mutation
Vesicular transport
Cell Biology
Golgi apparatus
Apical membrane
Transport protein
Cell biology
Vesicular transport protein
Protein Transport
Transmembrane region
symbols
biology.protein
lipids (amino acids, peptides, and proteins)
Influenza virus
Subjects
Details
- ISSN :
- 18733468
- Volume :
- 586
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- FEBS letters
- Accession number :
- edsair.doi.dedup.....1c7f2d0dc5f9ae11c06d311bc3fc2278