Additional file 1 of Cooperative treatment effectiveness of ATR and HSP90 inhibition in Ewing’s sarcoma cells

Additional file 1: Figure S1: Analysis of apoptosis and DDR signaling in ES cells. (A) Analysis p53 expression level by Western blot. β-actin was used to control protein loading. p53 null SaOS-2 cells were treated with the indicated concentrations of AUY922, VE821, KU55933 and their combinations. DMSO was used as control. (A-C) The loss of the mitochondrial transmembrane potential (ΔΨM) and cell death were assessed by flow cytometry after 48 h. All graphs show the mean ± SEM of three independent experiments. Statistical analysis was done using two-way ANOVA tests with GraphPad (*p