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Differences in the molecular signatures of mucosal-associated invariant T cells and conventional T cells
- Source :
- Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019), Scientific Reports
- Publication Year :
- 2019
- Publisher :
- Nature Publishing Group, 2019.
-
Abstract
- Mucosal-associated invariant T (MAIT) cells exhibit different characteristics from those of TCRα7.2− conventional T cells. They play important roles in various inflammatory diseases, including rheumatoid arthritis and inflammatory bowel disease. MAIT cells express a single T cell receptor alpha chain, TCRα7.2 segment associated with Jα33 and CDR3 with fixed length, which recognizes bacteria-derived vitamin B metabolites. However, the characteristics of MAIT cells and TCRα7.2+ CD161− T cells have never been compared. Here, we performed RNA sequencing to compare the properties of MAIT cells, TCRα7.2− conventional T cells and TCRα7.2+ CD161− T cells. Genome-wide transcriptomes of MAIT cells, TCRα7.2− conventional T cells, and TCRα7.2+ CD161− T cells were compared and analyzed using causal network analysis. This is the first report comparing the transcriptomes of MAIT cells, TCRα7.2− conventional T cells and TCRα7.2+ CD161− T cells. We also identified the predominant signaling pathways of MAIT cells, which differed from those of TCRα7.2− conventional T cells and TCRα7.2+ CD161− T cells, through a gene set enrichment test and upstream regulator analysis and identified the genes responsible for the characteristic MAIT cell phenotypes. Our study advances the complete understanding of MAIT biology.
- Subjects :
- 0301 basic medicine
Receptors, Antigen, T-Cell, alpha-beta
Cell
lcsh:Medicine
Blood Donors
Mucosal associated invariant T cell
Biology
Mucosal-Associated Invariant T Cells
Article
Gene regulatory networks
Arthritis, Rheumatoid
Transcriptome
03 medical and health sciences
0302 clinical medicine
Immunogenetics
medicine
T-Cell Receptor Alpha Chain
Humans
RNA-Seq
lcsh:Science
Gene
Multidisciplinary
lcsh:R
RNA
Molecular biology
Phenotype
Killer Cells, Natural
030104 developmental biology
medicine.anatomical_structure
Th17 Cells
lcsh:Q
Signal transduction
030217 neurology & neurosurgery
NK Cell Lectin-Like Receptor Subfamily B
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....1c6dd9914d183f6d35582bf357a69ec2