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Effect of selected B-ring-substituted oxysterols on artificial model erythrocyte membrane and isolated red blood cells
- Publication Year :
- 2020
-
Abstract
- In this paper, systematic studies concerning the influence of selected oxysterols on the structure and fluidity of human erythrocyte membrane modeled as Langmuir monolayers have been performed. Three oxidized cholesterol derivatives, namely 7α-hydroxycholesterol (7α-OH) 7β-hydroxycholesterol (7β-OH) and 7-ketocholesterol (7-K) have been incorporated in two different proportions (10 and 50%) into artificial erythrocyte membrane, modeled as two-component (cholesterol:POPC) Langmuir monolayer. All the studied oxysterols were found to alter membrane fluidity and the effect was more pronounced for higher oxysterol content. 7α-OH increased membrane fluidity while opposite effect was observed for 7β-OH and 7-K. Experiments performed on model systems have been verified in biological studies on red blood cells (RBC). Consistent results have been found, i.e. under the influence of 7α-OH, the elasticity of erythrocytes increased, and in the presence of other investigated oxysterols - decreased. The strongest effect was noticed for 7-K. Change of membrane elasticity was associated with the change of erythrocytes shape, being most noticeable under the influence of 7-K.
- Subjects :
- 0301 basic medicine
030103 biophysics
Langmuir
Erythrocytes
Oxysterol
Membrane Fluidity
Biophysics
01 natural sciences
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
0103 physical sciences
Monolayer
polycyclic compounds
Membrane fluidity
Humans
Elasticity (economics)
Cell Shape
Ketocholesterols
POPC
Cells, Cultured
010304 chemical physics
Cholesterol
Erythrocyte Membrane
Membranes, Artificial
Oxysterols
Cell Biology
Elasticity
Hydroxycholesterols
Membrane
chemistry
Phosphatidylcholines
lipids (amino acids, peptides, and proteins)
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....1c64950842b14504245914ce82f65165