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MiR-612 suppresses the stemness of liver cancer via Wnt/β-catenin signaling
- Source :
- Biochemical and biophysical research communications. 447(1)
- Publication Year :
- 2014
-
Abstract
- Previous research showed that microRNA-612 (miR-612) has inhibitory effects on cell proliferation, migration, invasion, and metastasis of hepatocellular carcinoma (HCC). AKT2 was confirmed to be a direct target of miR-612, through which the epithelial-mesenchymal transition (EMT) and metastasis of HCC were inhibited. Our present findings reveal that miR-612 is able to suppress the stemness of HCC by reducing the number and size of tumorspheres as well as clone formation in soft agar, and to relieve drug resistance to cisplatin and 5-fluorouracil. In addition, miR-612 hampered the capacity of tumorigenesis in NOD/SCID mice and redistributed the tumor invasive frontier of miR-612-modulating cells. Finally, our findings suggest that Wnt/β-catenin signaling is required in the regulation of EMT-associated stem cell-like traits by miR-612.
- Subjects :
- Male
Carcinoma, Hepatocellular
Epithelial-Mesenchymal Transition
Biophysics
Clone (cell biology)
AKT2
Mice, SCID
Biology
medicine.disease_cause
Biochemistry
Metastasis
Mice
Cancer stem cell
Mice, Inbred NOD
Cell Line, Tumor
medicine
Animals
Humans
Neoplasm Invasiveness
Molecular Biology
Wnt Signaling Pathway
beta Catenin
Cell Proliferation
Cell growth
Liver Neoplasms
Wnt signaling pathway
Cell Biology
Hep G2 Cells
medicine.disease
MicroRNAs
Drug Resistance, Neoplasm
Immunology
Cancer research
Neoplastic Stem Cells
Fluorouracil
Cisplatin
Carcinogenesis
Liver cancer
Neoplasm Transplantation
Subjects
Details
- ISSN :
- 10902104
- Volume :
- 447
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....1c5f6488935a1653fb8e2e1fab920790