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The Trouble with MEAM2: Implications of Pseudogenes on Species Delimitation in the Globally Invasive Bemisia tabaci (Hemiptera: Aleyrodidae) Cryptic Species Complex
- Source :
- Genome Biology and Evolution
- Publication Year :
- 2017
- Publisher :
- Oxford University Press (OUP), 2017.
-
Abstract
- Molecular species identification using suboptimal PCR primers can over-estimate species diversity due to coamplification of nuclear mitochondrial (NUMT) DNA/pseudogenes. For the agriculturally important whitefly Bemisia tabaci cryptic pest species complex, species identification depends primarily on characterization of the mitochondrial DNA cytochrome oxidase I (mtDNA COI) gene. The lack of robust PCR primers for the mtDNA COI gene can undermine correct species identification which in turn compromises management strategies. This problem is identified in the B. tabaci Africa/Middle East/Asia Minor clade which comprises the globally invasive Mediterranean (MED) and Middle East Asia Minor I (MEAM1) species, Middle East Asia Minor 2 (MEAM2), and the Indian Ocean (IO) species. Initially identified from the Indian Ocean island of Réunion, MEAM2 has since been reported from Japan, Peru, Turkey and Iraq. We identified MEAM2 individuals from a Peruvian population via Sanger sequencing of the mtDNA COI gene. In attempting to characterize the MEAM2 mitogenome, we instead characterized mitogenomes of MEAM1. We also report on the mitogenomes of MED, AUS, and IO thereby increasing genomic resources for members of this complex. Gene synteny (i.e., same gene composition and orientation) was observed with published B. tabaci cryptic species mitogenomes. Pseudogene fragments matching MEAM2 partial mtDNA COI gene exhibited low frequency single nucleotide polymorphisms that matched low copy number DNA fragments (
- Subjects :
- invasive pest
0301 basic medicine
NUMT
Species complex
Mitochondrial DNA
Letter
Pseudogene
Population
pseudogene
Biology
Synteny
DNA barcoding
Electron Transport Complex IV
Evolution, Molecular
Hemiptera
03 medical and health sciences
Genetics
Animals
DNA Barcoding, Taxonomic
education
Ecology, Evolution, Behavior and Systematics
education.field_of_study
Polymorphism, Genetic
mitogenome
Species diversity
H10 - Ravageurs des plantes
030104 developmental biology
high throughput -sequencing
Genome, Mitochondrial
Insect Proteins
Numt
Introduced Species
Pseudogenes
Subjects
Details
- ISSN :
- 17596653
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Genome Biology and Evolution
- Accession number :
- edsair.doi.dedup.....1c458663818ea59d0743f6c41dec2972