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Single-pulse CARS based multimodal nonlinear optical microscope for bioimaging

Single-pulse CARS based multimodal nonlinear optical microscope for bioimaging

Authors :
Angelika Unterhuber
Tschackad Kamali
Jonathan M. Levitte
Yaron Silberberg
René M. Werkmeister
Boris Považay
Wolfgang Drexler
Sunil Kumar
Boris Hermann
Ori Katz
Source :
Optics Express. 23:13082
Publication Year :
2015
Publisher :
The Optical Society, 2015.

Abstract

Noninvasive label-free imaging of biological systems raises demand not only for high-speed three-dimensional prescreening of morphology over a wide-field of view but also it seeks to extract the microscopic functional and molecular details within. Capitalizing on the unique advantages brought out by different nonlinear optical effects, a multimodal nonlinear optical microscope can be a powerful tool for bioimaging. Bringing together the intensity-dependent contrast mechanisms via second harmonic generation, third harmonic generation and four-wave mixing for structural-sensitive imaging, and single-beam/single-pulse coherent anti-Stokes Raman scattering technique for chemical sensitive imaging in the finger-print region, we have developed a simple and nearly alignment-free multimodal nonlinear optical microscope that is based on a single wide-band Ti:Sapphire femtosecond pulse laser source. Successful imaging tests have been realized on two exemplary biological samples, a canine femur bone and collagen fibrils harvested from a rat tail. Since the ultra-broad band-width femtosecond laser is a suitable source for performing high-resolution optical coherence tomography, a wide-field optical coherence tomography arm can be easily incorporated into the presented multimodal microscope making it a versatile optical imaging tool for noninvasive label-free bioimaging.

Details

ISSN :
10944087
Volume :
23
Database :
OpenAIRE
Journal :
Optics Express
Accession number :
edsair.doi.dedup.....1c308bc98ad159b9cbb1200dc576e2dc
Full Text :
https://doi.org/10.1364/oe.23.013082