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The Mammalian Cone Visual Cycle Promotes Rapid M/L-Cone Pigment Regeneration Independently of the Interphotoreceptor Retinoid-Binding Protein
- Source :
- The Journal of Neuroscience. 31:7900-7909
- Publication Year :
- 2011
- Publisher :
- Society for Neuroscience, 2011.
-
Abstract
- Rapid regeneration of the visual pigment following its photoactivation is critical for the function of cone photoreceptors throughout the day. Though the reactions of the visual cycle in the retinal pigment epithelium (RPE) that recycle chromophore for rod pigment regeneration are well characterized, the corresponding mechanisms that enable rapid regeneration of cone pigment are poorly understood. A key remaining question is the relative contribution of the recently discovered cone-specific retina visual cycle and the classic RPE-dependent visual cycle to mammalian cone pigment regeneration. In addition, it is not clear what role, if any, the abundant interphotoreceptor retinoid-binding protein (IRBP) presumed to facilitate the traffic of chromophore, plays in accelerating mammalian cone pigment regeneration. To address these issues, we used transretinal recordings to evaluate M/L-cone pigment regeneration in isolated retinas and eyecups from control and IRBP-deficient mice. Remarkably, the mouse retina promoted M/L-cone dark adaptation eightfold faster than the RPE. However, complete cone recovery required both visual cycles. We conclude that the retina visual cycle is critical for the initial rapid regeneration of mouse M/L-cone pigment during dark adaptation, whereas the slower RPE visual cycle is required to complete the process. While the deletion of IRBP reduced the amplitude and slowed the kinetics of mouse M/L-cone photoresponses, cone adaptation in bright, steady light and the kinetics of cone dark adaptation were not affected in isolated retina or in intact eyecup. Thus, IRBP does not accelerate cone pigment regeneration and is not critical for the function of mouse M/L-cones in bright light.
- Subjects :
- Male
Time Factors
genetic structures
Mice, Transgenic
Adaptation (eye)
Retinal Pigment Epithelium
Biology
Retinal Cone Photoreceptor Cells
Retina
Article
Mice
Pigment
Botany
medicine
Animals
Eye Proteins
Mice, Knockout
Mice, Inbred BALB C
Retinal pigment epithelium
General Neuroscience
Regeneration (biology)
Cone Opsins
Cone (formal languages)
eye diseases
Cell biology
Mice, Inbred C57BL
Retinol-Binding Proteins
medicine.anatomical_structure
visual_art
Visual Perception
visual_art.visual_art_medium
Female
sense organs
Retinal Pigments
Photic Stimulation
Visual phototransduction
Subjects
Details
- ISSN :
- 15292401 and 02706474
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- The Journal of Neuroscience
- Accession number :
- edsair.doi.dedup.....1c22272918f34a3228603ba6e2d37f7a