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Sensitive Determination Method for Mercury Ion, Methyl-, Ethyl-, and Phenyl-mercury in Water and Biological Samples Using High-Performance Liquid Chromatography with Chemiluminescence Detection
- Source :
- Analytical Sciences. 28:959-965
- Publication Year :
- 2012
- Publisher :
- Springer Science and Business Media LLC, 2012.
-
Abstract
- A sensitive determination method for mercury speciation analysis was developed. Four mercury species, mercury ion, methylmercury, ethylmercury, and phenylmercury, were complexed with emetine-dithiocarbamate (emetine-CS(2)), and then injected onto a HPLC instrument coupled with a tris(2,2'-bipyridine)ruthenium(III) chemiluminescence detection system. The emetine-CS(2) complexing agent was effectively used to measure the concentration in addition to serving as a separation and detection reagent. The calibration curves for these mercury complexes were linear in the range of 0.050 - 10 μg L(-1) (as Hg). The limit of detection for (emetine-CS(2))(2)Hg, emetine-CS(2)-methylmercury, emetine-CS(2)-ethylmercury, and emetine-CS(2)-phenylmercury were 30, 17, 21, and 22 ng L(-1), respectively. The sensitivity of this method enables the determination of mercury species in water samples at sub-ppb levels. Furthermore, the method was applied to biological samples in combination with acid leaching and liquid-liquid extraction using emetine-CS(2) as an extraction reagent. The determination results were in good agreement with the values of the certified reference materials.
- Subjects :
- Organomercury Compounds
Calibration curve
chemistry.chemical_element
High-performance liquid chromatography
Analytical Chemistry
law.invention
Ethylmercury
chemistry.chemical_compound
law
Animals
Humans
Chromatography, High Pressure Liquid
Ethylmercury Compounds
Chemiluminescence
Detection limit
Chromatography
Chemistry
Fishes
Water
Mercury
Methylmercury Compounds
Phenylmercury Compounds
Mercury (element)
Certified reference materials
Reagent
Luminescent Measurements
Environmental Pollutants
Hair
Subjects
Details
- ISSN :
- 13482246 and 09106340
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Analytical Sciences
- Accession number :
- edsair.doi.dedup.....1c1b6bb080410eb884292043f5a49097