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Anti-Fibrotic Effect of Synthetic Noncoding Oligodeoxynucleotide for Inhibiting mTOR and STAT3 via the Regulation of Autophagy in an Animal Model of Renal Injury
- Source :
- Molecules; Volume 27; Issue 3; Pages: 766, Molecules, Vol 27, Iss 766, p 766 (2022)
- Publication Year :
- 2022
- Publisher :
- MDPI AG, 2022.
-
Abstract
- Renal fibrosis is a common process of various kidney diseases. Autophagy is an important cell biology process to maintain cellular homeostasis. In addition, autophagy is involved in the pathogenesis of various renal disease, including acute kidney injury, glomerular diseases, and renal fibrosis. However, the functional role of autophagy in renal fibrosis remains poorly unclear. The mammalian target of rapamycin (mTOR) plays a negative regulatory role in autophagy. Signal transducer and activator of transcription 3 (STAT3) is an important intracellular signaling that may regulate a variety of inflammatory responses. In addition, STAT3 regulates autophagy in various cell types. Thus, we synthesized the mTOR/STAT3 oligodeoxynucleotide (ODN) to regulate the autophagy. The aim of this study was to investigate the beneficial effect of mTOR/STAT3 ODN via the regulation of autophagy appearance on unilateral ureteral obstruction (UUO)-induced renal fibrosis. This study showed that UUO induced inflammation, tubular atrophy, and tubular interstitial fibrosis. However, mTOR/STAT3 ODN suppressed UUO-induced renal fibrosis and inflammation. The autophagy markers have no statistically significant relation, whereas mTOR/STAT3 ODN suppressed the apoptosis in tubular cells. These results suggest the possibility of mTOR/STAT3 ODN for preventing renal fibrosis. However, the role of mTOR/STAT3 ODN on autophagy regulation needs to be further investigated.
- Subjects :
- STAT3 Transcription Factor
autophagy
urogenital system
TOR Serine-Threonine Kinases
oligodeoxynucleotide
antisense
Organic Chemistry
renal fibrosis
decoy
mTOR
STAT3
Pharmaceutical Science
Kidney
urologic and male genital diseases
Fibrosis
Analytical Chemistry
Disease Models, Animal
QD241-441
Oligodeoxyribonucleotides
Chemistry (miscellaneous)
Drug Discovery
Animals
Molecular Medicine
Physical and Theoretical Chemistry
Subjects
Details
- ISSN :
- 14203049
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....1c0f5775e48c54c68a5c9a9c54cad2eb