Additional file 4: of Pyruvate carboxylase supports the pulmonary tropism of metastatic breast cancer

Figure S4. PC is not required for cell migration. (A) Control (shScram) and PC depleted (shPC28) cells were plated into 8 μm transwell inserts in serum-free media containing 1 or 4 mM pyruvate. Cell migration was quantified 12 h after plating using calcein AM. SFM represents wells where the bottom chamber was filled with serum-free media as negative control. Values are presented as mean relative fluorescence units, ±SEM. (B) Control (shScram) and PC-depleted (shPC28) 4 T1 monolayers were wounded and closure was measured 15 and 24 h later. Values are presented as mean percent would closure, ±SEM. (PDF 372 kb)