Back to Search
Start Over
A human skin multifunctional O-acyltransferase that catalyzes the synthesis of acylglycerols, waxes, and retinyl esters
- Source :
- Journal of Lipid Research, Vol 46, Iss 11, Pp 2388-2397 (2005)
- Publication Year :
- 2005
- Publisher :
- Elsevier BV, 2005.
-
Abstract
- Acyl-CoA-dependent O-acyltransferases catalyze reactions in which fatty acyl-CoAs are joined to acyl acceptors containing free hydroxyl groups to produce neutral lipids. In this report, we characterize a human multifunctional O-acyltransferase (designated MFAT) that belongs to the acyl-CoA:diacylglycerol acyltransferase 2/acyl-CoA:monoacylglycerol acyltransferase (MGAT) gene family and is highly expressed in the skin. Membranes of insect cells and homogenates of mammalian cells overexpressing MFAT exhibited significantly increased MGAT, acyl-CoA:fatty acyl alcohol acyltransferase (wax synthase), and acyl-CoA:retinol acyltransferase (ARAT) activities, which catalyze the synthesis of diacylglycerols, wax monoesters, and retinyl esters, respectively. Furthermore, when provided with the appropriate substrates, intact mammalian cells overexpressing MFAT accumulated more waxes and retinyl esters than control cells. We conclude that MFAT is a multifunctional acyltransferase that likely plays an important role in lipid metabolism in human skin.
- Subjects :
- DNA, Complementary
Insecta
esterification
Glyceride
Immunoblotting
Fatty alcohol
QD415-436
Biology
Biochemistry
Catalysis
Article
Cell Line
Glycerides
chemistry.chemical_compound
Endocrinology
neutral lipids
Chlorocebus aethiops
Animals
Humans
Tissue Distribution
Diacylglycerol O-Acyltransferase
Cloning, Molecular
Phylogeny
Skin
Diacylglycerol kinase
Dose-Response Relationship, Drug
diacylglycerol
Retinol O-Fatty-Acyltransferase
Retinol
Esters
Lipid metabolism
Cell Biology
Blotting, Northern
Lipid Metabolism
Lipids
chemistry
Acyltransferases
Waxes
Acyltransferase
COS Cells
lipids (amino acids, peptides, and proteins)
fatty alcohol
Subjects
Details
- ISSN :
- 00222275
- Volume :
- 46
- Database :
- OpenAIRE
- Journal :
- Journal of Lipid Research
- Accession number :
- edsair.doi.dedup.....1c0652f7e9ffadaaac2335137066c9fa