Population Analysis of Susceptibility to Cefotaxime and Desacetyl-Cefotaxime in Staphylococcus and Enterobacteriaceae

Population analyses of susceptibility to cefotaxime (CTX) and desacetyl-cefotaxime (DCTX) of strains of Staphylococcus and some genera of Enterobacteriaceae were carried out. DCTX, which is the main metabolite of CTX, has antimicrobial activity. The penicillinase-producing strains of S. aureus and S. epidermidis were homogeneous as regards susceptibility to both agents. CTX was about 4-8 times more active than DCTX. The methicillin-resistant strains contained a sub-population of resistant bacteria with both CTX and DCTX. The frequency of resistant bacteria was 10(-6) - 10(-5). The E. coli strain was homogeneous to both agents. The strains of Enterobacter cloacae and Citrobacter freundii had a sub-population of resistant bacteria with both agents. The frequency of resistant bacteria was 10(-7) - 10(-4.5). In Klebsiella pneumoniae no resistant sub-population was found. CTX was about four times more active than DCTX with the strains of the Enterobacteriaceae. DCTX had no advantage over CTX as regards homogeneity of susceptibility of the populations examined. CTX seems applicable for treatment of infections with E. coli, Klebsiella pneumoniae, and penicillinase-producing, methicillin-susceptible Staphylococcus, but should not be used alone in treatments of infections with Enterobacter cloacae or Citrobacter freundii.