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Massively parallel sequencing of STRs using a 29-plex panel reveals stutter sequence characteristics
- Source :
- Electrophoresis5 References. 41(23)
- Publication Year :
- 2020
-
Abstract
- Massively parallel sequencing of forensic STRs simultaneously provides length-based genotypes and core repeat sequences as well as flanking sequence variations. Here, we report primer sequences and concentrations of a next-generation sequencing (NGS)-based in-house panel covering 28 autosomal STR loci (CSF1PO, D1GATA113, D1S1627, D1S1656, D1S1677, D2S441, D2S1776, D3S3053, D5S818, D6S474, D6S1017, D6S1043, D8S1179, D9S2157, D10S1435, D11S4463, D13S317, D14S1434, D16S539, D18S51, D18S853, D20S482, D20S1082, D22S1045, FGA, TH01, TPOX, and vWA) and the sex determinant locus Amelogenin. Preliminary evaluation experiments showed that the panel yielded intralocus- and interlocus-balanced sequencing data with a sensitivity as low as 62.5 pg input DNA. A total of 203 individuals from Yunnan Bai population were sequenced with this panel. Comparative forensic genetic analyses showed that sequence-based matching probability of this 29-plex panel reached 2.37 × 10-29 , which was 23 times lower than the length-based data. Compound stutter sequences of eight STRs were compared with parental alleles. For seven loci, repeat motif insertions or deletions occurred in the longest uninterrupted repeat sequences (LUS). However, LUS and non-LUS stutters co-existed in the locus D6S474 with different sequencing depth ratios. These results supplemented our current knowledge of forensic STR stutters, and provided a sound basis for DNA mixture deconvolution.
- Subjects :
- Forensic Genetics
China
Clinical Biochemistry
Population
Locus (genetics)
02 engineering and technology
Biology
01 natural sciences
Biochemistry
Deep sequencing
DNA sequencing
Analytical Chemistry
Asian People
Genotype
Humans
Allele
education
Genetics
education.field_of_study
Massive parallel sequencing
010401 analytical chemistry
High-Throughput Nucleotide Sequencing
Sequence Analysis, DNA
021001 nanoscience & nanotechnology
0104 chemical sciences
Microsatellite
0210 nano-technology
Multiplex Polymerase Chain Reaction
Microsatellite Repeats
Subjects
Details
- ISSN :
- 15222683
- Volume :
- 41
- Issue :
- 23
- Database :
- OpenAIRE
- Journal :
- Electrophoresis5 References
- Accession number :
- edsair.doi.dedup.....1bee75a8fc23f66b2cfb1a4f20d034c4