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Impaired hepatic counterregulatory response to insulin-induced hypoglycemia in hepatic denervated pigs
- Source :
- Nielsen, M F, Roelsgaard, K, Keiding, S, Brodersen, K, Møller, N, Vyberg, M & Vilstrup, H 2015, ' Impaired hepatic counterregulatory response to insulin-induced hypoglycemia in hepatic denervated pigs ', Journal of clinical & translational endocrinology, vol. 2, no. 4, pp. 131-136 . https://doi.org/10.1016/j.jcte.2015.08.004, Nielsen, M F, Roelsgaard, K, Keiding, S, Brodersen, K, Møller, N, Vyberg, M & Vilstrup, H 2015, ' Impaired hepatic counterregulatory response to insulin-induced hypoglycemia in hepatic denervated pigs ', Journal of Clinical & Translational Endocrinology, vol. 2, no. 4, pp. 131-136 . https://doi.org/10.1016/j.jcte.2015.08.004, Journal of Clinical & Translational Endocrinology, Vol 2, Iss 4, Pp 131-136 (2015), Journal of Clinical & Translational Endocrinology
- Publication Year :
- 2015
-
Abstract
- Highlights • Hepatic denervation results in a blunted counterregulatory response during insulin-induced hypoglycemia. • Fasting glucose concentration, glucose production and uptake are unaffected by hepatic denervation. • Insulin action and extrahepatic glucose uptake are unaffected by hepatic denervation.<br />Objective The liver reacts to hypoglycemia by increasing its glucose output. This response is assumed to depend both on glucose sensing at the liver and the brain, as well as efferent impulses from the brain to the liver. We tested the importance of this signaling pathway by studying the hepatic response to insulin-induced hypoglycemia in hepatic complete denervated pigs. Materials/methods Two weeks prior to the metabolic study, 36-kg pigs underwent either total hepatic denervation (DN; n = 12) or sham operation (sham; n = 12). On the metabolic study day, measurements were performed at baseline conditions and during a hypoglycemic hyperinsulinemic (5 mU/kg/min) clamp. Endogenous insulin and glucagon secretions were inhibited by somatostatin, and glucagon was replaced at baseline levels. Endogenous glucose production (EGP) and glucose utilization (Rd) were determined by [3-3H] glucose infusion. Results Baseline plasma concentrations of glucose, insulin, EGP and Rd did not differ significantly between the two groups of animals. During insulin infusion, the plasma glucose concentration was clamped at ~3 mmol/L in both groups of animals resulting in an increase in plasma concentrations of epinephrine and norepinephrine in sham pigs (both P
- Subjects :
- medicine.medical_specialty
Endogenous glucose production
Endocrinology, Diabetes and Metabolism
medicine.medical_treatment
Endogeny
Hypoglycemia
Glucagon
lcsh:Diseases of the endocrine glands. Clinical endocrinology
Norepinephrine (medication)
EGP, endogenous glucose production
Endocrinology
Internal medicine
medicine
Journal Article
Denervation
lcsh:RC648-665
business.industry
DN, denervated pigs
Insulin
medicine.disease
Hepatic innervation
Epinephrine
Somatostatin
Rd, rate of glucose utilization
business
Counterregulation
Research Paper
medicine.drug
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Nielsen, M F, Roelsgaard, K, Keiding, S, Brodersen, K, Møller, N, Vyberg, M & Vilstrup, H 2015, ' Impaired hepatic counterregulatory response to insulin-induced hypoglycemia in hepatic denervated pigs ', Journal of clinical & translational endocrinology, vol. 2, no. 4, pp. 131-136 . https://doi.org/10.1016/j.jcte.2015.08.004, Nielsen, M F, Roelsgaard, K, Keiding, S, Brodersen, K, Møller, N, Vyberg, M & Vilstrup, H 2015, ' Impaired hepatic counterregulatory response to insulin-induced hypoglycemia in hepatic denervated pigs ', Journal of Clinical & Translational Endocrinology, vol. 2, no. 4, pp. 131-136 . https://doi.org/10.1016/j.jcte.2015.08.004, Journal of Clinical & Translational Endocrinology, Vol 2, Iss 4, Pp 131-136 (2015), Journal of Clinical & Translational Endocrinology
- Accession number :
- edsair.doi.dedup.....1be2f8ca03f8b3eecfd1cf5f12643ddb