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Spatially patterned excitatory neuron subtypes and projections of the claustrum
- Source :
- eLife, Vol 10 (2021), eLife
- Publication Year :
- 2021
- Publisher :
- eLife Sciences Publications Ltd, 2021.
-
Abstract
- The claustrum is a functionally and structurally complex brain region, whose very spatial extent remains debated. Histochemical-based approaches typically treat the claustrum as a relatively narrow anatomical region that primarily projects to the neocortex, whereas circuit-based approaches can suggest a broader claustrum region containing projections to the neocortex and other regions. Here, in the mouse, we took a bottom-up and cell-type-specific approach to complement and possibly unite these seemingly disparate conclusions. Using single-cell RNA-sequencing, we found that the claustrum comprises two excitatory neuron subtypes that are differentiable from the surrounding cortex. Multicolor retrograde tracing in conjunction with 12-channel multiplexed in situ hybridization revealed a core-shell spatial arrangement of these subtypes, as well as differential downstream targets. Thus, the claustrum comprises excitatory neuron subtypes with distinct molecular and projection properties, whose spatial patterns reflect the narrower and broader claustral extents debated in previous research. This subtype-specific heterogeneity likely shapes the functional complexity of the claustrum.
- Subjects :
- Male
Mouse
QH301-705.5
Science
Short Report
Claustrum
Biology
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Mice
0302 clinical medicine
Cortex (anatomy)
Inhibitory neuron
Neural Pathways
medicine
Animals
Biology (General)
030304 developmental biology
Neurons
0303 health sciences
Neocortex
General Immunology and Microbiology
Sequence Analysis, RNA
General Neuroscience
cell type
General Medicine
Retrograde tracing
Mice, Inbred C57BL
Brain region
medicine.anatomical_structure
Medicine
Single-Cell Analysis
Spatial extent
Neuroscience
transcriptome
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- eLife
- Accession number :
- edsair.doi.dedup.....1bd5305d39c9d170c0eb06ef51254c32