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Spatially patterned excitatory neuron subtypes and projections of the claustrum

Authors :
Brianna N Bristow
Rennie M Kendrick
Kaitlin E Sullivan
Lihua Wang
Sarah R. Erwin
Brian A. Marriott
Jody Clements
Jesse Jackson
Mark S. Cembrowski
Andrew L. Lemire
Source :
eLife, Vol 10 (2021), eLife
Publication Year :
2021
Publisher :
eLife Sciences Publications Ltd, 2021.

Abstract

The claustrum is a functionally and structurally complex brain region, whose very spatial extent remains debated. Histochemical-based approaches typically treat the claustrum as a relatively narrow anatomical region that primarily projects to the neocortex, whereas circuit-based approaches can suggest a broader claustrum region containing projections to the neocortex and other regions. Here, in the mouse, we took a bottom-up and cell-type-specific approach to complement and possibly unite these seemingly disparate conclusions. Using single-cell RNA-sequencing, we found that the claustrum comprises two excitatory neuron subtypes that are differentiable from the surrounding cortex. Multicolor retrograde tracing in conjunction with 12-channel multiplexed in situ hybridization revealed a core-shell spatial arrangement of these subtypes, as well as differential downstream targets. Thus, the claustrum comprises excitatory neuron subtypes with distinct molecular and projection properties, whose spatial patterns reflect the narrower and broader claustral extents debated in previous research. This subtype-specific heterogeneity likely shapes the functional complexity of the claustrum.

Details

Language :
English
Volume :
10
Database :
OpenAIRE
Journal :
eLife
Accession number :
edsair.doi.dedup.....1bd5305d39c9d170c0eb06ef51254c32