Back to Search
Start Over
Haprin-deficient spermatozoa are incapable of in vitro fertilization
- Source :
- Molecular reproduction and developmentREFERENCES. 87(5)
- Publication Year :
- 2020
-
Abstract
- Haprin (TRIM36) is a ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. It is expressed in the testes in both mice and humans and is thought to be involved in spermiogenesis, the acrosome reaction, and fertilization. However, the functional role of Haprin is poorly understood. The aim of this study was to investigate the physiological role of Haprin in fertility. Homozygous haprin-deficient mice were generated and these mice, and their spermatozoa, were analyzed to detect morphological and fertility-related abnormalities. In these models, normal spermatogenesis was observed but sperm quality was reduced with haprin-deficient mice having poorer sperm morphology and motility than wild-type mice. Interestingly, haprin-deficient mice showed normal in vivo fertility but could not fertilize oocytes under standard in vitro fertilization conditions. In conclusion, this study demonstrated that Haprin deficiency causes morphological abnormalities in spermatozoa, indicating that Haprin is involved in spermiogenesis.
- Subjects :
- 0301 basic medicine
Male
Spermiogenesis
medicine.medical_treatment
Acrosome reaction
Motility
Fertilization in Vitro
Biology
Andrology
03 medical and health sciences
Mice
0302 clinical medicine
Human fertilization
In vivo
Genetics
medicine
Animals
Acrosome
Spermatogenesis
Infertility, Male
Mice, Knockout
030219 obstetrics & reproductive medicine
In vitro fertilisation
Acrosome Reaction
Seminal Plasma Proteins
Cell Biology
Spermatozoa
030104 developmental biology
Fertilization
Female
Carrier Proteins
Developmental Biology
Subjects
Details
- ISSN :
- 10982795
- Volume :
- 87
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Molecular reproduction and developmentREFERENCES
- Accession number :
- edsair.doi.dedup.....1bbe5c704c0c8181f5610d4dfc20fa37