Back to Search
Start Over
Increased activity of the complement system in the liver of patients with alcoholic hepatitis
- Source :
- Experimental and Molecular Pathology. 97:338-344
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- Inflammation has been suggested as a mechanism underlying the development of alcoholic hepatitis (AH). The activation of the complement system plays an important role in inflammation. Although it has been shown that ethanol-induced activation of the complement system contributes to the pathophysiology of ethanol-induced liver injury in mice, whether ethanol consumption activates the complement system in the human liver has not been investigated. Using antibodies against C1q, C3, and C5, the immunoreactivity of the complement system in patients with AH was examined by immunohistochemistry and quantified by morphometric image analysis. The immunoreactivity intensity of C1q, C3, and C5 in patients with AH was significantly higher than that seen in normal controls. Further, the gene expression of C1q, C3, and C5 was examined using real-time PCR. There were increases in the levels of C1q and C5, but not C3 mRNA in AH. Moreover, the immunoreactivity of C5a receptor (C5aR) also increased in AH. To explore the functional implication of the activation of the complement system in AH, we examined the colocalization of C5aR in Mallory-Denk bodies (MDBs) forming balloon hepatocytes. C5aR was focally overexpressed in the MDB forming cells. Collectively, our study suggests that alcohol consumption increases the activity of the complement system in the liver cells, which contributes to the inflammation-associated pathogenesis of AH.
- Subjects :
- medicine.medical_specialty
Clinical Biochemistry
Alcoholic hepatitis
Inflammation
Mallory Bodies
Real-Time Polymerase Chain Reaction
Biochemistry
Article
C5a receptor
Pathology and Forensic Medicine
Pathogenesis
Internal medicine
Genetics
medicine
Humans
Mallory body
Complement Activation
Molecular Biology
Liver injury
Ethanol
biology
Mechanism (biology)
Hepatitis, Alcoholic
business.industry
Complement System Proteins
medicine.disease
Immunohistochemistry
Complement system
Endocrinology
Immunology
biology.protein
medicine.symptom
Antibody
business
Biotechnology
Subjects
Details
- ISSN :
- 00144800
- Volume :
- 97
- Database :
- OpenAIRE
- Journal :
- Experimental and Molecular Pathology
- Accession number :
- edsair.doi.dedup.....1bb1ff1041dcf63a2151fbd27a0a747f