Back to Search
Start Over
β1-Syntrophin Modulation by miR-222 in mdx Mice
- Source :
- PLoS ONE, PLoS ONE, Vol 5, Iss 8 (2010)
- Publication Year :
- 2010
- Publisher :
- Public Library of Science, 2010.
-
Abstract
- BackgroundIn mdx mice, the absence of dystrophin leads to the deficiency of other components of the dystrophin-glycoprotein complex (DAPC), making skeletal muscle fibers more susceptible to necrosis. The mechanisms involved in the disappearance of the DAPC are not completely understood. The muscles of mdx mice express normal amounts of mRNA for the DAPC components, thus suggesting post-transcriptional regulation.Methodology/principal findingsWe investigated the hypothesis that DAPC reduction could be associated with the microRNA system. Among the possible microRNAs (miRs) found to be upregulated in the skeletal muscle tissue of mdx compared to wt mice, we demonstrated that miR-222 specifically binds to the 3'-UTR of β1-syntrophin and participates in the downregulation of β1-syntrophin. In addition, we documented an altered regulation of the 3'-UTR of β1-syntrophin in muscle tissue from dystrophic mice.Conclusion/significanceThese results show the importance of the microRNA system in the regulation of DAPC components in dystrophic muscle, and suggest a potential role of miRs in the pathophysiology of dystrophy.
- Subjects :
- Muscle tissue
Science
Down-Regulation
Mice, Transgenic
Neurological Disorders/Neuromuscular Diseases
Response Elements
Mice
Downregulation and upregulation
microRNA
Chlorocebus aethiops
medicine
Animals
Protein Isoforms
RNA, Messenger
Dystroglycans
Muscle, Skeletal
Molecular Biology
3' Untranslated Regions
Syntrophin
Glycoproteins
Regulation of gene expression
Multidisciplinary
biology
Dystrophy
Molecular biology
Dystrophin-associated protein
Cell biology
Up-Regulation
Muscular Dystrophy, Duchenne
MicroRNAs
medicine.anatomical_structure
Gene Expression Regulation
Molecular Biology/Post-Translational Regulation of Gene Expression
COS Cells
Dystrophin-Associated Proteins
biology.protein
Mice, Inbred mdx
Medicine
Dystrophin
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 5
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....1b91b67a74853347ef92d0a5e7f3eb88