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Improved liquid chromatographic method for the simultaneous determination of tramadol and its three main metabolites in human plasma, urine and saliva
- Source :
- Journal of Pharmaceutical and Biomedical Analysis. 44:1168-1173
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- Tramadol, an analgesic agent, and its main metabolites O -desmethyltramadol (M1), N -desmethyltramadol (M2) and O,N -didesmethyltramadol (M5) were determined simultaneously in human plasma, saliva and urine by a rapid and specific HPLC method. The sample preparation was a simple, one-step, extraction with ethyl acetate. Chromatographic separation was achieved with a Chromolith™ Performance RP-18e 100 mm × 4.6 mm column, using a mixture of methanol:water (19:81, v/v) adjusted to pH 2.5 by phosphoric acid, in an isocratic mode at flow rate of 2 ml/min. Fluorescence detection ( λ ex 200 nm/ λ em 301 nm) was used. The calibration curves were linear ( r 2 > 0.996) in the concentration ranges in plasma, saliva and urine. The lower limit of quantification was 2.5 ng/ml for all compounds. The within- and between-day precisions in the measurement of QC samples at four tested concentrations were acceptable in all analyzed body fluids The developed procedure was applied to assess the pharmacokinetics of tramadol and its main metabolites following administration of 100 mg single oral dose of tramadol to healthy volunteers.
- Subjects :
- Saliva
Time Factors
Monolithic HPLC column
Metabolite
Clinical Biochemistry
Ethyl acetate
Pharmaceutical Science
Urine
Sensitivity and Specificity
Analytical Chemistry
chemistry.chemical_compound
Pharmacokinetics
Drug Discovery
medicine
Humans
Phosphoric Acids
Sample preparation
Tramadol
Spectroscopy
Chromatography
Molecular Structure
Methanol
Reproducibility of Results
Water
Stereoisomerism
Hydrogen-Ion Concentration
Reference Standards
O-Desmethyltramadol
Analgesics, Opioid
Solutions
chemistry
Calibration
Chromatography, Liquid
Tablets
medicine.drug
Subjects
Details
- ISSN :
- 07317085
- Volume :
- 44
- Database :
- OpenAIRE
- Journal :
- Journal of Pharmaceutical and Biomedical Analysis
- Accession number :
- edsair.doi.dedup.....1b755fa767a3bd749b32d4d27422fb3b