Back to Search
Start Over
Proteomic demonstration of the recurrent presence of inter-alpha-inhibitor H4 heavy-chain during aspergillosis induced in an animal model
- Source :
- International journal of medical microbiology : IJMM, International journal of medical microbiology : IJMM, 2014, 304 (3-4), pp.327-38. ⟨10.1016/j.ijmm.2013.11.015⟩
- Publication Year :
- 2014
- Publisher :
- HAL CCSD, 2014.
-
Abstract
- International audience; Invasive pulmonary aspergillosis remains a matter of great concern in oncology/haematology, intensive care units and organ transplantation departments. Despite the availability of various diagnostic tools with attractive features, new markers of infection are required for better medical care. We therefore looked for potential pulmonary biomarkers of aspergillosis, by carrying out two-dimensional (2D) gel electrophoresis comparing the proteomes of bronchial-alveolar lavage fluids (BALF) from infected rats and from control rats presenting non-specific inflammation, both immunocompromised. A bioinformatic analysis of the 2D-maps revealed significant differences in the abundance of 20 protein spots (ANOVA P-value0.8). One of these proteins, identified by mass spectrometry, was considered of potential interest: inter-alpha-inhibitor H4 heavy-chain (ITIH4), characterised for the first time in this infectious context. Western blotting confirmed its overabundance in all infected BALF, particularly at early stages of murine aspergillosis. Further investigations were carried on rat serum, and confirmed that ITIH4 levels increased during experimental aspergillosis. Preliminary results in human samples strengthened this trend. To our knowledge, this is the first description of the involvement of ITIH4 in aspergillosis.
- Subjects :
- Male
Serum
Proteomics
Pathology
MESH: Rats, Sprague-Dawley
Aspergillosis
Invasive pulmonary aspergillosis
Organ transplantation
Rats, Sprague-Dawley
Two-dimensional gel electrophoresis
Electrophoresis, Gel, Two-Dimensional
MESH: Animals
0303 health sciences
Hematology
MESH: Serum
General Medicine
3. Good health
Blot
MESH: Alpha-Globulins
Infectious Diseases
Statistical analysis
Rat model
Bronchoalveolar Lavage Fluid
Microbiology (medical)
medicine.medical_specialty
Bronchial-alveolar lavage
Blotting, Western
Context (language use)
Biology
Microbiology
03 medical and health sciences
Intensive care
Internal medicine
Alpha-Globulins
medicine
Animals
MESH: Blotting, Western
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
MESH: Aspergillosis
030304 developmental biology
Mass spectrometry
030306 microbiology
MESH: Bronchoalveolar Lavage Fluid
MESH: Biological Markers
medicine.disease
MESH: Electrophoresis, Gel, Two-Dimensional
MESH: Male
Disease Models, Animal
Immunology
MESH: Disease Models, Animal
Biomarkers
Subjects
Details
- Language :
- English
- ISSN :
- 16180607
- Database :
- OpenAIRE
- Journal :
- International journal of medical microbiology : IJMM, International journal of medical microbiology : IJMM, 2014, 304 (3-4), pp.327-38. ⟨10.1016/j.ijmm.2013.11.015⟩
- Accession number :
- edsair.doi.dedup.....1b746289990198aa71b5a03b287e226c