Real-time assays for detection of Phytophthora spp. and identification of an avr3a gene variant

Authors :: Mahmoud M. A. Moustafa
Mariella M. Finetti Sialer
Aurelio Ciancio
Shimaa R. T. Tolba
Ibrahim I.S. EL-Shawaf
Chiara Ruggeri
Laura Cristina Rosso
Source :: Journal of plant diseases and protection (2006, Internet) 125 (2018): 331–337. doi:10.1007/s41348-018-0150-z, info:cnr-pdr/source/autori:Tolba S.R.T., Finetti Sialer M.M., Rosso L.C., Moustafa M.M.A., Ruggeri C., El-Shawaf I.I.S., Ciancio A./titolo:Real-time assays for detection of Phytophthora spp. and identification of an avr3a gene variant/doi:10.1007%2Fs41348-018-0150-z/rivista:Journal of plant diseases and protection (2006, Internet)/anno:2018/pagina_da:331/pagina_a:337/intervallo_pagine:331–337/volume:125
Publication Year :: 2018
Publisher :: Springer Science and Business Media LLC, 2018.
Abstract: A study was carried out for identification and detection of Phytophthora spp. from soil and plant samples, collected from Solanaceous crops in Egypt and Italy. The samples were screened with specific and universal primers of Phytophthora 18S, ITS1-ITS2 and 28S regions, followed by PCR product sequencing. The Phytophthora spp. detected were P. infestans (Egypt) and P. parasitica (Italy). A molecular beacon probe was also developed based on the avr3a gene of P. infestans to detect a variant associated with virulence traits. The probe was suitable for avr3a allele identification from P. infestans and also from P. parasitica PCR products.

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