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The emerging human pathogen Photorhabdus asymbiotica is a facultative intracellular bacterium and induces apoptosis of macrophage-like cells
- Source :
- Infection and Immunity, Infection and Immunity, American Society for Microbiology, 2009, 77 (3), pp.1022-1030. ⟨10.1128/IAI.01064-08⟩
- Publication Year :
- 2009
- Publisher :
- HAL CCSD, 2009.
-
Abstract
- Photorhabdus species are gram-negative entomopathogenic bacteria of the family Enterobacteriaceae . Among the different members of the genus, one species, Photorhabdus asymbiotica , is a pathogen of both insects and humans. The pathogenicity mechanisms of this bacterium are unknown. Here we show that P. asymbiotica is a facultative intracellular pathogen that is able to replicate inside human macrophage-like cells. Furthermore, P. asymbiotica was shown for the first time in an intracellular location after insect infection. We also demonstrated that among Australian and American clinical isolates, only the Australian strains were able to invade nonphagocytic human cells. In cell culture infection experiments, Australian clinical isolates as well as cell-free bacterial culture supernatant induced strong apoptosis of a macrophage cell line at 6 h postinfection. American isolates also induced cellular death, but much later than that induced by Australian ones. Mammalian cultured cells analyzed for key features of apoptosis displayed apoptotic nuclear morphology, activation of the initiator caspases 8 and 9 and the executioner caspases 3 and 7, and poly(ADP-ribose) polymerase proteolysis, suggesting activation of both the intrinsic and extrinsic apoptotic pathways.
- Subjects :
- Programmed cell death
CELL CULTURE
[SDV]Life Sciences [q-bio]
Immunology
MACROPHAGE-LIKE CELL
Human pathogen
Apoptosis
Microbiology
Microscopy, Electron, Transmission
Humans
PATHOGENICITY MECHANISM
Pathogen
RELATION HOTE-PARASITE
biology
Intracellular parasite
Macrophages
Enterobacteriaceae Infections
PATHOGEN INSECT
Bacterial Infections
PATHOGEN HUMAN
biology.organism_classification
Enterobacteriaceae
PHOTORHABDUS
Infectious Diseases
Cell culture
Parasitology
Photorhabdus
Subjects
Details
- Language :
- English
- ISSN :
- 00199567 and 10985522
- Database :
- OpenAIRE
- Journal :
- Infection and Immunity, Infection and Immunity, American Society for Microbiology, 2009, 77 (3), pp.1022-1030. ⟨10.1128/IAI.01064-08⟩
- Accession number :
- edsair.doi.dedup.....1b6e84bd047dd9515cc64e02ad40eff7