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Protein inhibitors of activated STAT resemble scaffold attachment factors and function as interacting nuclear receptor coregulators
- Source :
- The Journal of biological chemistry. 277(19)
- Publication Year :
- 2002
-
Abstract
- Protein inhibitor of activated STAT1 (PIAS1) functions as a nuclear receptor coregulator and is expressed in several cell types of human testis. However, the mechanism of PIAS1 coregulation is unknown. We report here that PIAS1 has characteristics of a scaffold attachment protein. PIAS1 localized in nuclei in a speckled pattern and bound A-T-rich double-stranded DNA, a function of scaffold attachment proteins in chromatin regions of active transcription. DNA binding was dependent on a 35-amino acid sequence conserved among members of the PIAS family and in scaffold attachment proteins. The PIAS family also bound the androgen receptor DNA binding domain, and binding required the second zinc finger of this domain. PIAS1 contained an intrinsic activation domain but had bi-directional effects on androgen receptor transactivation; lower expression levels inhibited and higher levels increased transactivation in CV1 cells. Other PIAS family members also had dose-dependent effects on transactivation, but they were in a direction opposite to those of PIAS1. When coexpressed with PIAS1, other PIAS family members counteracted PIAS1 coregulation of androgen receptor transactivation. The interaction of PIAS1 with other members of the PIAS family suggests a transcription coregulatory mechanism involving a multicomponent PIAS nuclear scaffold.
- Subjects :
- Male
Transcriptional Activation
Transcription, Genetic
Molecular Sequence Data
Nuclear receptor coregulators
Biology
Transfection
Biochemistry
Cell Line
Transactivation
Mice
Two-Hybrid System Techniques
Testis
Animals
Humans
Protein inhibitor of activated STAT
Amino Acid Sequence
Luciferases
Molecular Biology
In Situ Hybridization
Zinc finger
Cell Nucleus
Dose-Response Relationship, Drug
Sequence Homology, Amino Acid
Age Factors
Proteins
Cell Biology
DNA-binding domain
DNA
Oligonucleotides, Antisense
Blotting, Northern
beta-Galactosidase
Molecular biology
Immunohistochemistry
Protein Inhibitors of Activated STAT
Chromatin
Cell biology
Protein Structure, Tertiary
Androgen receptor
Nuclear receptor
Receptors, Androgen
COS Cells
Plasmids
Protein Binding
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 277
- Issue :
- 19
- Database :
- OpenAIRE
- Journal :
- The Journal of biological chemistry
- Accession number :
- edsair.doi.dedup.....1b618577be0988103350272c35f5b6c6