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Production of transgenic pigs over-expressing the antiviral gene Mx1
- Source :
- Cell Regeneration, Cell Regeneration, Vol 3, Iss 1 (2014)
- Publication Year :
- 2014
- Publisher :
- BioMed Central, 2014.
-
Abstract
- The myxovirus resistance gene (Mx1) has a broad spectrum of antiviral activities. It is therefore an interesting candidate gene to improve disease resistance in farm animals. In this study, we report the use of somatic cell nuclear transfer (SCNT) to produce transgenic pigs over-expressing the Mx1 gene. These transgenic pigs express approximately 15–25 times more Mx1 mRNA than non-transgenic pigs, and the protein level of Mx1 was also markedly enhanced. We challenged fibroblast cells isolated from the ear skin of transgenic and control pigs with influenza A virus and classical swine fever virus (CFSV). Indirect immunofluorescence assay (IFA) revealed a profound decrease of influenza A proliferation in Mx1 transgenic cells. Growth kinetics showed an approximately 10-fold reduction of viral copies in the transgenic cells compared to non-transgenic controls. Additionally, we found that the Mx1 transgenic cells were more resistant to CSFV infection in comparison to non-transgenic cells. These results demonstrate that the Mx1 transgene can protect against viral infection in cells of transgenic pigs and indicate that the Mx1 transgene can be harnessed to develop disease-resistant pigs.
- Subjects :
- Somatic cell nuclear transfer
lcsh:R5-920
biology
Transgene
Research
Innate resistance
Cell Biology
biology.organism_classification
medicine.disease_cause
Virology
Virus
Green fluorescent protein
lcsh:Biology (General)
Classical swine fever
Influenza A virus
medicine
Stem cell
lcsh:Medicine (General)
Gene
lcsh:QH301-705.5
Developmental Biology
Antiviral breeding
Subjects
Details
- Language :
- English
- ISSN :
- 20459769
- Volume :
- 3
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Cell Regeneration
- Accession number :
- edsair.doi.dedup.....1b53ab106f54c10059165d5975611334