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Zebrafish (Danio rerio) larvae as a model for real-time studies of propagating VHS virus infection, tissue tropism and neutrophil activity
- Source :
- Journal of Fish Diseases, Journal of Fish Diseases, Wiley, 2021, 44 (5), pp.563-571. ⟨10.1111/jfd.13294⟩
- Publication Year :
- 2020
-
Abstract
- International audience; Viral haemorrhagic septicaemia virus (VHSV) is a negative-sense single-stranded RNA virus that infects more than 140 different fish species. In this study, zebrafish larvae were employed as in vivo model organisms to investigate progression of disease, the correlation between propagation of the infection and irreversibility of disease, cell tropism and in situ neutrophil activity towards the VHSV-infected cells. A recombinant VHSV strain, encoding "tomato" fluorescence (rVHSV-Tomato), was used in zebrafish to be able to follow the progress of the infection in the live host in real-time. Two-day-old zebrafish larvae were injected into the yolk sac with the recombinant virus. The virus titre peaked 96 hr post-infection in zebrafish larvae kept at 18 degrees C, and correlated with 33% mortality and high morbidity among the larvae. By utilizing the transgenic zebrafish line Tg(fli1:GFP)(y1) with fluorescently tagged endothelial cells, we were able to demonstrate that the virus initially infected endothelial cells lining the blood vessels. By observing the rVHSV-Tomato infection in the neutrophil reporter zebrafish line Tg(MPX:eGFP)(i114) , we inferred that only a subpopulation of the neutrophils responded to the virus infection. We conclude that the zebrafish larvae are suitable for real-time studies of VHS virus infections, allowing in vivo dissection of host-virus interactions at the whole organism level.
- Subjects :
- 0301 basic medicine
in vivo visualization
neutrophil response
animal structures
Neutrophils
Veterinary (miscellaneous)
ved/biology.organism_classification_rank.species
Aquatic Science
Recombinant virus
Tropism
Virus
Green fluorescent protein
Novirhabdovirus
03 medical and health sciences
viral haemorrhagic septicaemia virus
Hemorrhagic Septicemia, Viral
Animals
Model organism
Zebrafish
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
biology
ved/biology
fungi
tissue tropism
RNA virus
04 agricultural and veterinary sciences
biology.organism_classification
Virology
mortality
Disease Models, Animal
030104 developmental biology
040102 fisheries
Tissue tropism
0401 agriculture, forestry, and fisheries
infection kinetics
Subjects
Details
- ISSN :
- 13652761 and 01407775
- Volume :
- 44
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Journal of fish diseasesREFERENCES
- Accession number :
- edsair.doi.dedup.....1b495f281801eb7d93dcb0fce8784a99