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Clinical value of microRNA-378a-3p in sepsis and its role in sepsis-induced inflammation and cardiac dysfunction
- Source :
- Bioengineered, Vol 12, Iss 1, Pp 8496-8504 (2021), Bioengineered, article-version (VoR) Version of Record
- Publication Year :
- 2021
- Publisher :
- Informa UK Limited, 2021.
-
Abstract
- This study explored the clinical meaning of miR-378a-3p in sepsis and its influence on sepsis-induced inflammation and cardiac dysfunction. Serum levels of miR-378a-3p were detected by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR). The Receiver Operating Characteristic (ROC) curve was used to evaluate its diagnostic value. The effects of miR-378a-3p on inflammation and cardiac function were evaluated by monitoring left ventricular systolic pressure (LVSP), left ventricular and end-diastolic pressure (LVEDP), maximum rate of change in left ventricular pressure (± dp/dtmax) and detecting the levels of troponin I (cTnI), creatine kinase isoenzyme MB (CK-MB), tumor necrosis factor-a (TNF-a), interleukin-6 (IL-6), and interleukin-1β (IL-1β) via enzyme linked immunosorbent assay (ELISA). Serum miR-378a-3p was increased in sepsis patients and rat model. ROC curve indicated that miR-378a-3p might have diagnostic significance for sepsis miR-378a-3p antagomir improved the cardiac function by upregulating the levels of LVSP and ± dp/dtmax, and decreasing the levels of LVEDP, cTnI and CK-MB in rat model. miR-378a-3p antagomir also significantly alleviated the inflammatory responseby down-regulating the expression of TNF-a, IL-6, and IL-1β. Besides, logistics regression analysis illustrated that miR-378a-3p was an independent influencing factor for the onset of cardiac dysfunction in sepsis. miR-378a-3p has the potential as a diagnostic biomarker for sepsis and decreasing the level of miR-378a-3p had the ability to ameliorate cardiac dysfunction and inflammatory response caused by sepsis.
- Subjects :
- Adult
Male
Cardiac function curve
medicine.medical_specialty
mir-378a-3p
diagnosis
Bioengineering
Inflammation
Applied Microbiology and Biotechnology
Rats, Sprague-Dawley
sepsis
Sepsis
Internal medicine
Troponin I
medicine
Animals
Humans
Aged
business.industry
General Medicine
Middle Aged
medicine.disease
Rats
MicroRNAs
Preload
cardiovascular system
Cardiology
Ventricular pressure
Cytokines
biomarker
Biomarker (medicine)
Female
Tumor necrosis factor alpha
medicine.symptom
Cardiomyopathies
business
TP248.13-248.65
Research Article
Research Paper
Biotechnology
Subjects
Details
- ISSN :
- 21655987 and 21655979
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Bioengineered
- Accession number :
- edsair.doi.dedup.....1b46569b2e5632a9a0643bfc8ba67ee4