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Proteomic Analysis of Human Blister Fluids Following Envenomation by Three Snake Species in India: Differential Markers for Venom Mechanisms of Action
- Source :
- Toxins, Toxins, Vol 11, Iss 5, p 246 (2019), Volume 11, Issue 5
- Publication Year :
- 2019
-
Abstract
- Skin blistering as a result of snakebite envenomation is characteristic of some bites, however little is known regarding the mechanism of blister formation or the composition of the blister fluid. In order to investigate if blister fluid proteomes from humans suffering snakebite envenomation could provide insights on the pathophysiology of these skin alterations, blister fluid was collected from six patients upon presentation at a clinic in India bitten by three species of snakes, Daboia russelii (3), Hypnale hypnale (2), or Naja naja (1). Standard clinical data were recorded throughout the treatment. Approximately 805 proteins were identified in blister fluids using proteomic analyses. Informatics analyses of the proteomes identified the top biological response categories as: platelet degranulation, innate immune response, receptor-mediated endocytosis, complement activation, and blood coagulation. Hierarchical clustering did not show a clear segregation of patients&rsquo<br />proteomes being associated with the species of snake involved, suggesting that either the proteomic profiles described reflect a general response to venom-induced tissue damage or more patient data sets will be required to observe significant differences. Finally, it is of interest that venom proteins were also identified in the blister fluids suggesting that this fluid may serve as a reservoir of venom biologically active proteins/toxins, and as such, may indicate the clinical value of removing blister fluid to attenuate further tissue damage.
- Subjects :
- Male
Proteomics
Proteome
Health, Toxicology and Mutagenesis
wound exudate
lcsh:Medicine
Snake Bites
Venom
Reptilian Proteins
Toxicology
0302 clinical medicine
Blister
Platelet degranulation
skin and connective tissue diseases
snake venom
0303 health sciences
biology
integumentary system
Snakes
Middle Aged
Snake venom
Child, Preschool
Female
medicine.symptom
Adult
blister fluid
Naja
extracellular matrix
030231 tropical medicine
India
Inflammation
Viper Venoms
Article
03 medical and health sciences
Young Adult
medicine
Animals
Humans
snake venom metalloproteinases (SVMP)
Envenomation
030304 developmental biology
Aged
Elapid Venoms
Innate immune system
lcsh:R
Infant
biology.organism_classification
inflammation
Immunology
Subjects
Details
- ISSN :
- 20726651
- Volume :
- 11
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Toxins
- Accession number :
- edsair.doi.dedup.....1b30db8ed010e2bca02c000fc4f57ae1