Back to Search
Start Over
Anti-inflammatory effects of N-cyclooctyl-5-methylthiazol-2-amine hydrobromide on lipopolysaccharide-induced inflammatory response through attenuation of NLRP3 activation in microglial cells
- Source :
- BMB Reports
- Publication Year :
- 2021
- Publisher :
- Korean Society for Biochemistry and Molecular Biology, 2021.
-
Abstract
- Microglial activation is closely associated with neuroinflammatory pathologies. The nucleotide-binding and oligomerization domain-like receptor containing a pyrin domain 3 (NLRP3) inflammasomes are highly organized intracellular sensors of neuronal alarm signaling. NLRP3 inflammasomes activate nuclear factor kappa-B (NF-κB) and reactive oxygen species (ROS), which induce inflammatory responses. Moreover, NLRP3 dysfunction is a common feature of chronic inflammatory diseases. The present study investigated the effect of a novel thiazol derivative, N-cyclooctyl-5-methylthiazol-2-amine hydrobromide (KHG26700), on inflammatory responses in lipopolysaccharide (LPS)-treated BV-2 microglial cells. KHG26700 significantly attenuated the expression of several pro-inflammatory cytokines, including tumor necrosis factor-α, interleukin-1β, and interleukin-6, in these cells, as well as the LPS-induced increases in NLRP3, NF-κB, and phospho-IkBα levels. KHG26700 also suppressed the LPS-induced increases in protein levels of autophagy protein 5 (ATG5), microtubule- associated protein 1 light chain 3 (LC3), and beclin-1, as well as downregulating the LPS-enhanced levels of ROS, lipid peroxidation, and nitric oxide. These results suggest that the anti-inflammatory effects of KHG26700 may be due, at least in part, to the regulation of the NLRP3-mediated signaling pathway during microglial activation. [BMB Reports 2021; 54(11): 557-562].
- Subjects :
- Bromides
Lipopolysaccharides
Lipopolysaccharide
Inflammasomes
ATG5
Pro-inflammatory cytokines
Anti-Inflammatory Agents
Nitric Oxide
Biochemistry
Pyrin domain
Article
Nitric oxide
Inflammasome
chemistry.chemical_compound
Mice
NLRP3
NLR Family, Pyrin Domain-Containing 3 Protein
Animals
Molecular Biology
chemistry.chemical_classification
Inflammation
Reactive oxygen species
Tumor Necrosis Factor-alpha
NF-kappa B
General Medicine
Macrophage Activation
Cell biology
chemistry
Autophagy Protein 5
Cytokines
Tumor necrosis factor alpha
Microglia
Lipid Peroxidation
Signal transduction
Reactive Oxygen Species
N-cyclooctyl-5-methylthiazol-2-amine hydrobromide
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 1976670X and 19766696
- Volume :
- 54
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- BMB Reports
- Accession number :
- edsair.doi.dedup.....1b1cf8d9abbfcf9217836757172764b5