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Protection against heat-induced cell killing by alanine
- Source :
- International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group. 4(3)
- Publication Year :
- 1988
-
Abstract
- When L-alanine was added either to full growth medium or to Hanks' balanced salt solution (HBSS) prior to hyperthermia, survival of heated cells was significantly increased in a concentration-dependent manner. Maximal heat protection was not immediate, but required at least 1 h at 37 degrees C incubation prior to heating. Heat protection was principally reflected in an increased Dq on the 45 degrees C survival curve; for example, with 100 mM L-alanine, the Dq increased from approximately equal to 20 (control) to 30 min at 45 degrees C. Hyperthermia of 1 h at temperatures between 42 degrees C and 45 degrees C indicated that 100 mM alanine had shifted the isotoxic temperature by 0.5 degrees C. Comparable heat protection was also observed with D-alanine and amino acid dimers, such as alanyl-alanine or alanyl-leucine. Leucine at similar concentrations by itself, without alanine, did not protect cells against heat killing, but increased cellular heat sensitivity. The data suggest that heat protection by alanine does not require incorporation of alanine into cellular protein, but is mediated by the free amino acid.
- Subjects :
- Hyperthermia
Cancer Research
Hot Temperature
Time Factors
Physiology
Cell Survival
Balanced salt solution
Biology
chemistry.chemical_compound
Cricetulus
Physiology (medical)
Cricetinae
medicine
Animals
Incubation
Cells, Cultured
Alanine
chemistry.chemical_classification
Growth medium
Cytotoxins
Ovary
medicine.disease
Amino acid
Cell killing
chemistry
Biochemistry
Biophysics
Female
Leucine
Subjects
Details
- ISSN :
- 02656736
- Volume :
- 4
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group
- Accession number :
- edsair.doi.dedup.....1b1c4e23460d95cd34adcf64f1a971cf