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Expression of Prostatic Acid Phosphatase in Rat Circumvallate Papillae
- Source :
- PLoS ONE, Vol 11, Iss 6, p e0158401 (2016), PLoS ONE
- Publication Year :
- 2016
- Publisher :
- Public Library of Science (PLoS), 2016.
-
Abstract
- ATP and its metabolites are important for taste signaling in taste buds, and thus a clearance system for them would play critical roles in maintenance of gustatory function. A previous report revealed that mRNAs for ecto-5'-nucleotidase (NT5E) and prostatic acid phosphatase (PAP) were expressed by taste cells of taste buds, and NT5E-immunoreactivity was detected in taste cells. However, there was no information on PAP-immunoreactivity in taste buds. In this study, we examined the expression profile of PAP in rat taste buds. In the isolated rat taste buds, we detected expression of mRNA for PAP, but NT5E was not detected differing from the case of mouse ones (Dando et al., 2012, J Neuroscience). On immunohistochemical analysis, PAP-immunoreactivity was found predominantly in NTPDase2-positive type I and SNAP25-positive type III taste cells, while there were no apparent signals of it in PLC-β2-positive type II, α-gustducin-positive type II, AADC-positive type III and 5HT-positive type III ones. As for NT5E, we could not detect its immunoreactivity in rat taste buds, and co-localization of it with any taste cell markers, although mouse taste buds expressed NT5E as reported previously. These findings suggest that PAP expressed by type I and one of type III taste cells of rats may contribute to metabolic regulation of the extracellular levels of adenine nucleotides in the taste buds of circumvallate papillae, and the regulating mechanisms for adenine nucleotides in taste buds might be different between rats and mice.
- Subjects :
- 0301 basic medicine
Male
Taste
Sensory Receptors
Social Sciences
Gene Expression
lcsh:Medicine
Artificial Gene Amplification and Extension
Immunostaining
Biochemistry
Polymerase Chain Reaction
5'-nucleotidase
Mice
0302 clinical medicine
TAS1R3
TAS1R2
Adenine nucleotide
Taste receptor
Psychology
Lingual papilla
lcsh:Science
5'-Nucleotidase
Staining
Multidisciplinary
biology
Nucleotides
Cell Staining
Taste Buds
Immunohistochemistry
Sensory Perception
Research Article
Signal Transduction
medicine.medical_specialty
Acid Phosphatase
Research and Analysis Methods
03 medical and health sciences
Internal medicine
medicine
Animals
RNA, Messenger
Molecular Biology Techniques
Immunohistochemistry Techniques
Molecular Biology
Adenine
lcsh:R
Acid phosphatase
Biology and Life Sciences
Cell Biology
Rats
Histochemistry and Cytochemistry Techniques
Nuclear Staining
030104 developmental biology
Endocrinology
Specimen Preparation and Treatment
biology.protein
Immunologic Techniques
lcsh:Q
030217 neurology & neurosurgery
Neuroscience
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....1b1abaae49431b7607ea2a32c265aec4