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Change in small intestinal brush border membranes of rats following methotrexate administration
- Source :
- Pharmacologytoxicology. 65(4)
- Publication Year :
- 1989
-
Abstract
- Change in small intestinal brush border membranes of rats following methotrexate administration was monitored by the fluorescence spectra and polarization of the cationic fluorescent probe, safranin 0. Total protein content of brush border membranes of treated rats was less than that of control rats, whereas no significant difference in total sialic acid content of brush border membranes was observed between them. Thus, an apparent increase in the electronegative charge of brush border membrane vesicles per unit of membrane protein following methotrexate administration may possibly cause the change in fluorescence spectra and polarization of safranin 0.
- Subjects :
- Male
medicine.medical_specialty
Brush border
Health, Toxicology and Mutagenesis
Fluorescence spectrometry
Toxicology
chemistry.chemical_compound
Oral administration
Internal medicine
Safranin
Intestine, Small
medicine
Animals
Pharmacology
Microvilli
Vesicle
Rats, Inbred Strains
Sialic acid
Rats
Endocrinology
Membrane
Methotrexate
Spectrometry, Fluorescence
chemistry
Membrane protein
Biophysics
Spectrophotometry, Ultraviolet
Subjects
Details
- ISSN :
- 09019928
- Volume :
- 65
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Pharmacologytoxicology
- Accession number :
- edsair.doi.dedup.....1aee5a8d44c3f43196f54bbe9c528801