Back to Search
Start Over
Molecular Characterization of Seizure-Related Genes Isolated by Differential Screening
- Source :
- Biochemical and Biophysical Research Communications. 219:795-799
- Publication Year :
- 1996
- Publisher :
- Elsevier BV, 1996.
-
Abstract
- To isolate seizure-related genes, we applied differential screening technique to the cDNA library which constructed from primary cultured cerebral cortical cells treated with pentylenetetrazol (PTZ). Northern blotting analysis of mRNA levels in the cerebra after systemic administration of PTZ confirmed the results of differential screening procedure: 6 clones showed increased mRNA level and 3 clones showed decreased expression with PTZ. Interestingly, 4 genes which were isolated by this technique were related to intracellular calcium action. They were cytosolic phospholipase A2, 78 kDa glucose regulated protein, SEZ-15 which has an EF hand motif and PTZ-17 that causes calcium current in Xenopus oocyte with PTZ application. These data and our previous results suggest that intracellular calcium may play an important role for seizure-related pathophysiological changes in neuronal cells.
- Subjects :
- Male
DNA, Complementary
Xenopus
Molecular Sequence Data
Biophysics
Gene Expression
Sequence Homology
chemistry.chemical_element
Biology
Calcium
Biochemistry
Phospholipases A
Calcium in biology
Mice
Seizures
Gene expression
medicine
Animals
Amino Acid Sequence
Northern blot
Cloning, Molecular
Pentylenetetrazol
Endoplasmic Reticulum Chaperone BiP
Molecular Biology
Cells, Cultured
Heat-Shock Proteins
Neurons
cDNA library
Genes, fos
Cell Biology
biology.organism_classification
Molecular biology
Recombinant Proteins
Mice, Inbred C57BL
Phospholipases A2
Cytosol
chemistry
Oocytes
Pentylenetetrazole
Female
Carrier Proteins
Proto-Oncogene Proteins c-fos
Molecular Chaperones
medicine.drug
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 219
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....1ad9b8beccf660c7c84fcffa5b7bc442