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Effects of Ethanol and Its Metabolites on Human Pancreatic Stellate Cells
- Source :
- Digestive Diseases and Sciences. 55:204-211
- Publication Year :
- 2009
- Publisher :
- Springer Science and Business Media LLC, 2009.
-
Abstract
- Pancreatic stellate cells (PSCs) play a pivotal role in pancreatic inflammation and fibrosis. In the pancreas, in addition to oxidative metabolism, ethanol can be metabolized by esterification with fatty acids to form fatty acid ethyl esters such as palmitic acid ethyl ester (PAEE). We here examined the effects of ethanol (at 20 or 50 mM), acetaldehyde (at 200 microM), or PAEE (at 100 microM), on PSCs functions. PSCs did not express mRNAs for pancreatic triglyceride lipase and carboxyl ester lipase. Ethanol and acetaldehyde, but not PAEE, induced production of procollagen type I C-peptide. Ethanol, but not acetaldehyde or PAEE, induced interleukin-8 production. PAEE activated activator protein-1, but not nuclear factor kappaB. In addition, PAEE activated extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38 mitogen-activated protein kinase. Specific activation of signal transduction pathways and cell functions by ethanol and its metabolites may play a role in alcohol-induced pancreatic injury.
- Subjects :
- medicine.medical_specialty
Physiology
Palmitates
Acetaldehyde
Palmitic acid
chemistry.chemical_compound
Internal medicine
medicine
Humans
Lipase
Protein kinase A
Pancreas
Cells, Cultured
Cell Proliferation
Mitogen-Activated Protein Kinase Kinases
chemistry.chemical_classification
Triglyceride lipase
Ethanol
biology
Interleukin-8
NF-kappa B
Gastroenterology
Fatty acid
Peptide Fragments
Transcription Factor AP-1
Endocrinology
chemistry
Biochemistry
biology.protein
Hepatic stellate cell
Collagen
Procollagen
Subjects
Details
- ISSN :
- 15732568 and 01632116
- Volume :
- 55
- Database :
- OpenAIRE
- Journal :
- Digestive Diseases and Sciences
- Accession number :
- edsair.doi.dedup.....1ac10d75a539a6f95e6124cc4fc1e7d6