Back to Search Start Over

Sec22b-dependent antigen cross-presentation is a significant contributor of T cell priming during infection with the parasite Trypanosoma cruzi

Authors :
Biscari, Lucía
Maza, Ma Carmen
Farré, Cecilia
Kaufman, Cintia Daniela
Amigorena, Sebastian
Fresno Escudero, Manuel
Gironès, Núria
Alloatti, Andrés
UAM. Departamento de Biología Molecular
Source :
Frontiers in Cell and Developmental Biology. 11
Publication Year :
2023
Publisher :
Frontiers Media SA, 2023.

Abstract

Antigen cross-presentation is a vital mechanism of dendritic cells and other antigen presenting cells to orchestrate the priming of cytotoxic responses towards killing of infected or cancer cells. In this process, exogenous antigens are internalized by dendritic cells, processed, loaded onto MHC class I molecules and presented to CD8+ T cells to activate them. Sec22b is an ER-Golgi Intermediate Compartment resident SNARE protein that, in partnership with sintaxin4, coordinates the recruitment of the transporter associated with antigen processing protein and the peptide loading complex to phagosomes, where antigenic peptides that have been proteolyzed in the cytosol are loaded in MHC class I molecules and transported to the cell membrane. The silencing of Sec22b in dendritic cells primary cultures and conditionally in dendritic cells of C57BL/6 mice, critically impairs antigen cross-presentation, but neither affects other antigen presentation routes nor cytokine production and secretion. Mice with Sec22b conditionally silenced in dendritic cells (Sec22b−/− ) show deficient priming of CD8+ T lymphocytes, fail to control tumor growth, and are resistant to anti-checkpoint immunotherapy. In this work, we show that Sec22b−/− mice elicit a deficient specific CD8+ T cell response when challenged with sublethal doses of Trypanosoma cruzi trypomastigotes that is associated with increased blood parasitemia and diminished survival<br />This research was funded by grants from “Ministerio de Ciencia e Innovación” (SAF 2016–75988-R. PID-2019-104760RB-100), “Comunidad de Madrid (S2017/BMD-3671. INFLAMUNE-CM) to MF “Ministerio de Ciencia, Innovación y Universidades Agencia Estatal de Investigación and Fondo Europeo de Desarrollo Regional (PGC 2018–096132-B-I00 and PID 2021–123389OB-I00 (MICINN/FEDER) to NG CBMSO institutional grants from “Fundación Ramón Areces” and “Banco de Santander” are also acknowledged. AA was suported by the Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación of Argentina through the grants: PICT 2018–4664, PICT 2017–1367, PICT 2020-SERIEA-01643. AA was also supported by Fundación Carolina and the Ministerio de Educación de la República Argentina

Details

ISSN :
2296634X
Volume :
11
Database :
OpenAIRE
Journal :
Frontiers in Cell and Developmental Biology
Accession number :
edsair.doi.dedup.....1ab2e7e6cfa8884f167498ea1469e220
Full Text :
https://doi.org/10.3389/fcell.2023.1138571