Back to Search
Start Over
Relationship between knockdown resistance, metabolic detoxification and organismal resistance to pyrethroids in Anopheles sinensis
- Source :
- PLoS ONE, Vol 8, Iss 2, p e55475 (2013), Zhong, D; Chang, X; Zhou, G; He, Z; Fu, F; Yan, Z; et al.(2013). Relationship between Knockdown Resistance, Metabolic Detoxification and Organismal Resistance to Pyrethroids in Anopheles sinensis. PLoS ONE, 8(2). doi: 10.1371/journal.pone.0055475. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/3fr32757, PLoS ONE
- Publication Year :
- 2013
- Publisher :
- Public Library of Science (PLoS), 2013.
-
Abstract
- Anopheles sinensis is the most important vector of malaria in Southeast Asia, including China. Currently, the most effective measure to prevent malaria transmission relies on vector control through the use of insecticides, primarily pyrethroids. Extensive use of insecticides poses strong selection pressure on mosquito populations for resistance. Resistance to insecticides can arise due to mutations in the insecticide target site (target site resistance), which in the case of pyrethroids is the para-type sodium channel gene, and/or the catabolism of the insecticide by detoxification enzymes before it reaches its target (metabolic detoxification resistance). In this study, we examined deltamethrin resistance in An. sinensis from China and investigated the relative importance of target site versus metabolic detoxification mechanisms in resistance. A high frequency (>85%) of nonsynonymous mutations in the para gene was found in populations from central China, but not in populations from southern China. Metabolic detoxification as measured by the activity of monooxygenases and glutathione S-transferases (GSTs) was detected in populations from both central and southern China. Monooxygenase activity levels were significantly higher in the resistant than the susceptible mosquitoes, independently of their geographic origin. Stepwise multiple regression analyses in mosquito populations from central China found that both knockdown resistance (kdr) mutations and monooxygenase activity were significantly associated with deltamethrin resistance, with monooxygenase activity playing a stronger role. These results demonstrate the importance of metabolic detoxification in pyrethroid resistance in An. sinensis, and suggest that different mechanisms of resistance could evolve in geographically different populations. © 2013 Zhong et al.
- Subjects :
- Insecticides
Mosquito Control
Epidemiology
ved/biology.organism_classification_rank.species
lcsh:Medicine
Mosquitoes
Mixed Function Oxygenases
Insecticide Resistance
chemistry.chemical_compound
0302 clinical medicine
Pyrethrins
lcsh:Science
Genome Evolution
Glutathione Transferase
Genetics
0303 health sciences
Multidisciplinary
Anopheles
Agriculture
Genomics
3. Good health
Mosquito control
Gene Knockdown Techniques
Inactivation, Metabolic
Research Article
China
Genotype
030231 tropical medicine
Aedes aegypti
Biology
Microbiology
Vector Biology
Anopheles sinensis
Molecular Genetics
03 medical and health sciences
Genetic Mutation
Detoxification
Nitriles
parasitic diseases
medicine
Animals
Pesticides
030304 developmental biology
Evolutionary Biology
Population Biology
business.industry
ved/biology
lcsh:R
Knockdown resistance
medicine.disease
biology.organism_classification
Biotechnology
Insect Vectors
Deltamethrin
Culicidae
chemistry
Mutation
Genetics of Disease
Genetic Polymorphism
Parasitology
lcsh:Q
Pest Control
Population Ecology
Gene Function
business
Zoology
Malaria
Population Genetics
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....1aaa48c035f83f3806bdc111110a168b