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Neutrophils Do Not Express IL-17A in the Context of Acute Oropharyngeal Candidiasis
- Source :
- Pathogens, Vol 4, Iss 3, Pp 559-572 (2015), Pathogens
- Publication Year :
- 2015
- Publisher :
- MDPI AG, 2015.
-
Abstract
- IL-17 protects against pathogens by acting on nonhematopoietic cells to induce neutrophil recruitment through upregulation of chemokines and G-CSF. IL-17- and Th17-deficient humans and mice are susceptible to mucosal Candida albicans infections, linked to impaired neutrophil responses. IL-17 production is traditionally associated with CD4+ Th17 cells. However, IL-17 is also expressed during innate responses to facilitate rapid pathogen clearance. Innate IL-17-expressing cells include various lymphocyte-type subsets, including ILC3, NKT, γδ-T and “natural” Th17 (nTh17) cells. Some reports suggest that neutrophils can express IL-17 during fungal infections. Here, we asked whether neutrophils serve as a source of IL-17 during acute oropharyngeal candidiasis (OPC) using an IL-17A fate-tracking reporter mouse. Mice were subjected to OPC for two days, and oral tissue was analyzed by flow cytometry. IL-17A was expressed by γδ-T cells and TCRβ+ natural Th17 (nTh17) cells, as recently reported. Although infiltrating neutrophils were recruited to the tongue following infection, they did not express the IL-17A reporter. Moreover, neutrophil-depleted mice exhibited normal transcription of both Il17a and downstream IL-17-dependent gene targets after Candida challenge. Thus, in acute OPC, neutrophils are not a measurable source of IL-17 production, nor are they necessary to trigger IL-17-dependent gene expression, although they are essential for ultimate pathogen control.
- Subjects :
- Microbiology (medical)
Chemokine
mice
γδ-T cell
lcsh:Medicine
Oropharyngeal Candidiasis
Flow cytometry
Microbiology
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
neutrophils
Candida albicans
medicine
oral candidiasis
Immunology and Allergy
Molecular Biology
Pathogen
030304 developmental biology
0303 health sciences
General Immunology and Microbiology
biology
medicine.diagnostic_test
Brief Report
fungal infection
lcsh:R
biology.organism_classification
3. Good health
IL-17
Infectious Diseases
Immunology
biology.protein
IL17A
Interleukin 17
Th17
innate lymphocyte
TCR
030215 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 20760817
- Volume :
- 4
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Pathogens
- Accession number :
- edsair.doi.dedup.....1a94e1420546c0d5a9cd7e738adb7159