Back to Search
Start Over
A Subanesthetic Dose of Isoflurane during Postconditioning Ameliorates Zymosan-Induced Neutrophil Inflammation Lung Injury and Mortality in Mice
- Source :
- Mediators of Inflammation, Mediators of Inflammation, Vol 2013 (2013)
- Publication Year :
- 2013
- Publisher :
- Hindawi Limited, 2013.
-
Abstract
- Anesthetic isoflurane (ISO) has immunomodulatory effects. In the present study, we investigated whether a subanesthetic dose of ISO (0.7%) protected against zymosan (ZY) induced inflammatory responses in the murine lung and isolated neutrophils. At 1 and 6 hrs after ZY administration intraperitoneally, ISO was inhaled for 1 hr, and 24 hrs later, lung inflammation and injury were assessed. We found that ISO improved the survival rate of mice and mitigated lung injury as characterized by the histopathology, wet-to-dry weight ratio, protein leakage, and lung function index. ISO significantly attenuated ZY-induced lung neutrophil recruitment and inflammation. This was suggested by the downregulation of (a) endothelial adhesion molecule expression and myeloperoxidase (MPO) activity in lung tissue and polymorphonuclear neutrophils (b) chemokines, and (c) proinflammatory cytokines in BALF. Furthermore, ZY-induced nuclear translocation and DNA-binding activity of NF-κB p65 were also reduced by ISO. ISO treatment inhibited iNOS expression and activity, as well as subsequent nitric oxide generation. Consistent with thesein vivoobservations,in vitrostudies confirmed that ISO blocked NF-κB and iNOS activation in primary mouse neutrophils challenged by ZY. These results provide evidence that 0.7% ISO ameliorates inflammatory responses in ZY-treated mouse lung and primary neutrophils.
- Subjects :
- Male
Chemokine
Time Factors
Article Subject
Neutrophils
Immunology
Active Transport, Cell Nucleus
Down-Regulation
Nitric Oxide Synthase Type II
Inflammation
Lung injury
Pharmacology
Nitric oxide
Proinflammatory cytokine
Mice
chemistry.chemical_compound
In vivo
lcsh:Pathology
medicine
Animals
Lung
Nitrites
Peroxidase
Mice, Inbred BALB C
Isoflurane
biology
business.industry
Zymosan
NF-kappa B
Lung Injury
Pneumonia
Cell Biology
Hydrogen-Ion Concentration
chemistry
Myeloperoxidase
biology.protein
Cytokines
Blood Gas Analysis
Chemokines
medicine.symptom
business
Bronchoalveolar Lavage Fluid
lcsh:RB1-214
Research Article
Subjects
Details
- ISSN :
- 14661861 and 09629351
- Volume :
- 2013
- Database :
- OpenAIRE
- Journal :
- Mediators of Inflammation
- Accession number :
- edsair.doi.dedup.....1a69567e484af9a0d3b9222bfc47704c