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Schistosome infectivity in the snail, Biomphalaria glabrata, is partially dependent on the expression of Grctm6, a Guadeloupe Resistance Complex protein
- Source :
- PLoS Neglected Tropical Diseases, Vol 11, Iss 2, p e0005362 (2017), PLoS Neglected Tropical Diseases
- Publication Year :
- 2017
- Publisher :
- Public Library of Science (PLoS), 2017.
-
Abstract
- Schistosomiasis is one of the most important neglected tropical diseases. Despite effective chemotherapeutic treatments, this disease continues to afflict hundreds of millions of people. Understanding the natural intermediate snail hosts of schistosome parasites is vital to the suppression of this disease. A recently identified genomic region in Caribbean Biomphalaria glabrata snails strongly influences their resistance to infection by Schistosoma mansoni. This region contains novel genes having structural similarity to known pathogen recognition proteins. Here we elaborate on the probable structure and role of one of these genes, grctm6. We characterised the expression of Grctm6 in a population of Caribbean snails, and performed a siRNA knockdown of Grctm6. We show that this protein is not only expressed in B. glabrata hemolymph, but that it also has a role in modulating the number of S. mansoni cercariae released by infected snails, making it a possible target for the biological control of schistosomiasis.<br />Author summary Schistosomiasis is one of the most prevalent parasitic diseases in the world. Though treatments for schistosomiasis infection exist, there is no vaccine, and reinfection is common in areas where the parasite occurs. One possible way to mitigate schistosomiasis is by controlling the transmission of the parasite larvae from the snails that carry them. Understanding the snail-parasite relationship is essential for the development of new means to interrupt transmission of the parasite from snails to humans. Snails possess immune mechanisms for fighting infection, most of which are based in hemolymph tissue. Here we characterize a novel protein, Grctm6, in a snail host of schistosome parasites. Grctm6 is structurally similar to certain other immune proteins and is present in snail hemolymph. Importantly, we demonstrate that the release of schistosomes by infected snails is exacerbated when this protein is experimentally suppressed in live snails. These results support the suitability of Grctm6 as a possible target for reducing the transmission of this human disease.
- Subjects :
- 0301 basic medicine
Physiology
Snails
Pathogenesis
Snail
Disease Vectors
Molting
Pathology and Laboratory Medicine
Biochemistry
0302 clinical medicine
Medicine and Health Sciences
Schistosomiasis
Small interfering RNAs
Pathogen
Infectivity
education.field_of_study
Biomphalaria
biology
Ecology
lcsh:Public aspects of medicine
Helminth Proteins
Schistosoma mansoni
3. Good health
Nucleic acids
Infectious Diseases
Caribbean Region
Helminth Infections
Host-Pathogen Interactions
Schistosoma
Research Article
Neglected Tropical Diseases
lcsh:Arctic medicine. Tropical medicine
lcsh:RC955-962
030231 tropical medicine
Population
Microbiology
03 medical and health sciences
Protein Domains
Helminths
biology.animal
parasitic diseases
Genetics
Parasitic Diseases
medicine
Animals
Biomphalaria glabrata
Gene Silencing
Non-coding RNA
education
Gene Expression Profiling
fungi
Organisms
Public Health, Environmental and Occupational Health
Biology and Life Sciences
Proteins
lcsh:RA1-1270
Molluscs
Tropical Diseases
biology.organism_classification
medicine.disease
Invertebrates
Gene regulation
030104 developmental biology
Gastropods
RNA
Gene expression
Physiological Processes
Subjects
Details
- Language :
- English
- ISSN :
- 19352735 and 19352727
- Volume :
- 11
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- PLoS Neglected Tropical Diseases
- Accession number :
- edsair.doi.dedup.....1a675d936dbbeba328a4a986738e5414