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Synechocystis 6803 mutants expressing distinct forms of the Photosystem II D1 protein from Synechococcus 7942: relationship between the psbA coding region and sensitivity to visible and UV-B radiation
- Source :
- Biochimica et biophysica acta. 1605(1-3)
- Publication Year :
- 2003
-
Abstract
- Synechocystis PCC 6803 mutants expressing either the “low light” (D1:1) or the “high light” (D1:2) form of the Photosystem II (PSII) D1 protein from Synechococcus PCC 7942 were constructed and characterized with respect to properties of PSII and sensitivity to visible and UV-B radiation. The AI and AIII mutants (containing only the D1:1 and D1:2 forms, respectively) exhibited very similar PSII characteristics as the control strain and they differed only in the accelerated decay kinetics of flash-induced variable fluorescence measured in the presence of DCMU. However, the mutants showed increased sensitivity to photodamage induced by visible and UV-B radiation, with higher loss of PSII activity in the AI than in the AIII strain. Thus, the difference between strains containing D1:1 and D1:2 found previously in Synechococcus 7942 is maintained after transfer of corresponding psbA genes into Synechocystis 6803 and is directly related to the coding region of these genes. The higher light sensitivity of the AI mutant is caused partly by the higher rate of photodamage and partly by the less efficient PSII repair.
- Subjects :
- Photoinhibition
Photosystem II
Light
Ultraviolet Rays
Mutant
Photosynthetic Reaction Center Complex Proteins
Biophysics
D1 protein
macromolecular substances
Photochemistry
Photosynthesis
Cyanobacteria
Biochemistry
Synechocystis PCC 6803
chemistry.chemical_compound
Structure-Activity Relationship
Synechococcus PCC 7942
biology
Synechocystis
Photosystem II Protein Complex
DCMU
Cell Biology
Synechococcus
biology.organism_classification
Fluorescence
Phenotype
chemistry
Mutation
Subjects
Details
- ISSN :
- 00063002
- Volume :
- 1605
- Issue :
- 1-3
- Database :
- OpenAIRE
- Journal :
- Biochimica et biophysica acta
- Accession number :
- edsair.doi.dedup.....1a4f1f06ba550b07e1b1c7e0bd5cafb9