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Transcriptomic Analysis of Mating Responses in Bemisia tabaci MED Females

Authors :
Bao-Ping Pang
Shaoli Wang
Wen Xie
Qingjun Wu
Jinjian Yang
Xuguo Zhou
Yating Liu
Youjun Zhang
Zhijia Huo
Source :
Insects, Volume 11, Issue 5, Insects, Vol 11, Iss 308, p 308 (2020)
Publication Year :
2020
Publisher :
Multidisciplinary Digital Publishing Institute, 2020.

Abstract

Mating triggers substantial changes in gene expression and leads to subsequent physiological and behavioral modifications. However, postmating transcriptomic changes responding to mating have not yet been fully understood. Here, we carried out RNA sequencing (RNAseq) analysis in the sweet potato whitefly, Bemisia tabaci MED, to identify genes in females in response to mating. We compared mRNA expression in virgin and mated females at 24 h. As a result, 434 differentially expressed gene transcripts (DEGs) were identified between the mated and unmated groups, including 331 up- and 103 down-regulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that many of these DEGs encode binding-related proteins and genes associated with longevity. An RT-qPCR validation study was consistent with our transcriptomic analysis (14/15). Specifically, expression of P450s (Cyp18a1 and Cyp4g68), ubiquitin-protein ligases (UBR5 and RNF123), Hsps (Hsp68 and Hsf), carboxylase (ACC-2), facilitated trehalose transporters (Tret1-2), transcription factor (phtf), and serine-protein kinase (TLK2) were significantly elevated in mated females throughout seven assay days. These combined results offer a glimpe of postmating molecular modifications to facilitate reproduction in B. tabaci females.

Details

Language :
English
ISSN :
20754450
Database :
OpenAIRE
Journal :
Insects
Accession number :
edsair.doi.dedup.....1a326bb20034b940681e81e1949e5cd7
Full Text :
https://doi.org/10.3390/insects11050308