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Fluorescent Tobacco mosaic virus-derived bio-nanoparticles for intravital two-photon imaging
- Source :
- Frontiers in Plant Science, Frontiers in Plant Science, Frontiers, 2016, 6, pp.1244-1244, Frontiers in Plant Science, Vol 6 (2016)
- Publication Year :
- 2016
- Publisher :
- HAL CCSD, 2016.
-
Abstract
- Multi-photon intravital imaging has become a powerful tool to investigate the healthy and diseased brain vasculature in living animals. Although agents for multi-photon fluorescence microscopy of the microvasculature are available, issues related to stability, bioavailability, toxicity, cost or chemical adaptability remain to be solved. In particular, there is a need for highly fluorescent dyes linked to particles that do not cross the blood brain barrier (BBB) in brain diseases like tumor or stroke to estimate the functional blood supply. Plant virus particles possess a number of distinct advantages over other particles, the most important being the multi-valency of chemically addressable sites on the particle surface. This multi-valency, together with biological compatibility and inert nature, makes plant viruses ideal carriers for in vivo imaging agents. Here, we show that the well-known Tobacco mosaic virus is a suitable nanocarrier for two-photon dyes and for intravital imaging of the mouse brain vasculature.
- Subjects :
- 0301 basic medicine
Plant virus
02 engineering and technology
Plant Science
Biology
lcsh:Plant culture
Blood–brain barrier
03 medical and health sciences
Two-photon excitation microscopy
Tobacco mosaic virus
medicine
Fluorescence microscope
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
[SDV.BV] Life Sciences [q-bio]/Vegetal Biology
lcsh:SB1-1110
two-photon microscopy
Original Research
viral nanoparticles
021001 nanoscience & nanotechnology
Virology
Fluorescence
030104 developmental biology
medicine.anatomical_structure
Biophysics
intravital imaging
Nanocarriers
0210 nano-technology
Preclinical imaging
Subjects
Details
- Language :
- English
- ISSN :
- 1664462X
- Database :
- OpenAIRE
- Journal :
- Frontiers in Plant Science, Frontiers in Plant Science, Frontiers, 2016, 6, pp.1244-1244, Frontiers in Plant Science, Vol 6 (2016)
- Accession number :
- edsair.doi.dedup.....1a2d882202691802fd690ec318aa211b