Back to Search
Start Over
Osteogenic Differentiation of Mesenchymal Stromal Cells: A Comparative Analysis Between Human Subcutaneous Adipose Tissue and Dental Pulp
- Source :
- Stem Cells and Development. 26:843-855
- Publication Year :
- 2017
- Publisher :
- Mary Ann Liebert Inc, 2017.
-
Abstract
- White adipose tissue is a source of mesenchymal stromal/stem cells (MSCs) that are actively studied for their possible therapeutic use in bone tissue repair/remodeling. To better appreciate the osteogenic potential of these cells, we compared some properties of MSCs from human subcutaneous adipose tissue [subcutaneous-adipose stromal cells (S-ASCs)] and dental pulp stem cell (DPSCs) of third-impacted molars, the latter representing a well-established MSC source. Both undifferentiated cell types showed similar fibroblast-like morphology and mesenchymal marker expression. However, undifferentiated S-ASCs displayed a faster doubling time coupled to greater proliferation and colony-forming ability than DPSCs. Also, the osteogenic differentiation of S-ASCs was greater than that of DPSCs, as evaluated by the higher levels of expression of early osteogenic markers Runt-related transcription factor-2 (RUNX2) and alkaline phosphatase at days 3-14 and of extracellular matrix mineralization at days 14-21. Moreover, S-ASCs showed a better colonization of the titanium scaffold. In addition, we investigated whether S-ASC osteogenic commitment was enhanced by adenosine A1 receptor (A1R) stimulation, as previously shown for DPSCs. Although A1R expression was constant during DPSC differentiation, it increased in S-ASC at day 21 from osteogenesis induction. Accordingly, A1R stimulation by the agonist 2-chloro-N6-cyclopentyl-adenosine, added to the cultures at each medium change, stimulated proliferation only in differentiating DPSC and enhanced the osteogenic differentiation earlier in DPSCs than in S-ASCs. These effects were counteracted by cell pretreatment with a selective A1R antagonist. Thus, our findings suggest that S-ASCs could be advantageously used in regenerative orthopedics/dentistry, and locally released or exogenously added purines may play a role in bone repair/remodeling, even though this aspect should be more thoroughly evaluated.
- Subjects :
- Male
0301 basic medicine
Adenosine
Stromal cell
Adolescent
Subcutaneous Fat
White adipose tissue
Biology
Bone tissue
Regenerative medicine
Extracellular matrix
03 medical and health sciences
stomatognathic system
Osteogenesis
medicine
Humans
Cell Lineage
Cell Shape
Dental Pulp
Cell Proliferation
Receptor, Adenosine A1
Mesenchymal stem cell
Spectrometry, X-Ray Emission
Cell Differentiation
Mesenchymal Stem Cells
Cell Biology
Hematology
Anatomy
Cell biology
RUNX2
Phenotype
030104 developmental biology
medicine.anatomical_structure
Female
Stem cell
Developmental Biology
Subjects
Details
- ISSN :
- 15578534 and 15473287
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Stem Cells and Development
- Accession number :
- edsair.doi.dedup.....1a29b36e2084aa4c1beefd5130551920