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Line differences in Cor/Lea and fructan biosynthesis-related gene transcript accumulation are related to distinct freezing tolerance levels in synthetic wheat hexaploids
Line differences in Cor/Lea and fructan biosynthesis-related gene transcript accumulation are related to distinct freezing tolerance levels in synthetic wheat hexaploids
- Source :
- Journal of plant physiology. 176
- Publication Year :
- 2014
-
Abstract
- In common wheat, cultivar differences in freezing tolerance are considered to be mainly due to allelic differences at two major loci controlling freezing tolerance. One of the two loci, Fr-2, is coincident with a cluster of genes encoding C-repeat binding factors (CBFs), which induce downstream Cor/Lea genes during cold acclimation. Here, we conducted microarray analysis to study comprehensive changes in gene expression profile under long-term low-temperature (LT) treatment and to identify other LT-responsive genes related to cold acclimation in leaves of seedlings and crown tissues of a synthetic hexaploid wheat line. The microarray analysis revealed marked up-regulation of a number of Cor/Lea genes and fructan biosynthesis-related genes under the long-term LT treatment. For validation of the microarray data, we selected four synthetic wheat lines that contain the A and B genomes from the tetraploid wheat cultivar Langdon and the diverse D genomes originating from different Aegilops tauschii accessions with distinct levels of freezing tolerance after cold acclimation. Quantitative RT-PCR showed increased transcript levels of the Cor/Lea, CBF, and fructan biosynthesis-related genes in more freezing-tolerant lines than in sensitive lines. After a 14-day LT treatment, a significant difference in fructan accumulation was observed among the four lines. Therefore, the fructan biosynthetic pathway is associated with cold acclimation in development of wheat freezing tolerance and is another pathway related to diversity in freezing tolerance, in addition to the CBF-mediated Cor/Lea expression pathway.
- Subjects :
- Physiology
Plant Science
Quantitative trait locus
Genes, Plant
Polyploidy
Fructan
Gene Expression Regulation, Plant
Gene expression
Freezing
Cold acclimation
Aegilops tauschii
RNA, Messenger
Common wheat
Gene
Triticum
Oligonucleotide Array Sequence Analysis
Plant Proteins
Genetics
biology
Microarray analysis techniques
food and beverages
biology.organism_classification
Adaptation, Physiological
Fructans
Up-Regulation
Plant Leaves
Solubility
Seedlings
Agronomy and Crop Science
Subjects
Details
- ISSN :
- 16181328
- Volume :
- 176
- Database :
- OpenAIRE
- Journal :
- Journal of plant physiology
- Accession number :
- edsair.doi.dedup.....1a22e4d89da2fa41ad22bfd4a755a8d9