Back to Search
Start Over
Flow Cytometric Analysis of Circadian Changes in Platelet Activation Using Anti-Gmp-140 Monoclonal Antibody
- Source :
- Chronobiology International. 16:335-342
- Publication Year :
- 1999
- Publisher :
- Informa UK Limited, 1999.
-
Abstract
- The hemostatic activity of blood shows a circadian variation with a higher frequency of acute coronary events in the morning. The thrombotic tendency of blood is influenced by many factors, including platelets. Diurnal changes of in vivo platelet activation were investigated by whole blood flow cytometry in 10 young healthy male volunteers using anti-GMP-140 (anti-alpha-granule membrane protein 140 kD) monoclonal antibody at 3h intervals from 06:00 to 24:00. We also studied circulating platelet aggregates to investigate whether there exists a similarity between the results of these methods. Results of flow cytometric analysis indicate that there is an increase in platelet activation during the period from 06:00 to 09:00. Platelet activation then decreases gradually during the period from noon to midnight. These changes are accompanied by a similar trend in circulating platelet aggregates. This suggests that GMP-140 expression on platelets is synchronized with or followed by platelet aggregate formation in vivo, and increased platelet activation may predispose individuals to thrombosis at this time.
- Subjects :
- Adult
Male
medicine.medical_specialty
Platelet Aggregation
Physiology
medicine.drug_class
Biology
Monoclonal antibody
Flow cytometry
In vivo
Physiology (medical)
Internal medicine
medicine
Humans
Platelet
Platelet activation
Circadian rhythm
Whole blood
Morning
medicine.diagnostic_test
Antibodies, Monoclonal
Flow Cytometry
Platelet Activation
Circadian Rhythm
P-Selectin
Endocrinology
Platelet Glycoprotein GPIb-IX Complex
Subjects
Details
- ISSN :
- 15256073 and 07420528
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Chronobiology International
- Accession number :
- edsair.doi.dedup.....1a14fb9ede8ee1191facc8973f3c6d19