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Induction of IFN-gamma and IL-1 alpha production in macrophages stimulated with phosphopolysaccharide produced by Lactococcus lactis ssp. cremoris
- Source :
- International journal of food microbiology. 31(1-3)
- Publication Year :
- 1996
-
Abstract
- The induction of interferon (IFN) and interleukin-1 (IL-1) production in murine macrophages by a phosphopolysaccharide, produced by a dairy lactic acid bacteria, Lactococcus lactis ssp. cremoris, was investigated. When the phosphopolysaccharide was added into macrophage cultures at concentrations from 1 to 200 micrograms/ml, substantial IFN titers (6.2-79.2 IU/ml) were detected. Using the reverse transcription-polymerase chain reaction (RT-PCR), the expression of mRNA encoding IFN-gamma was verified in spleen macrophage cultures. Macrophages stimulated with the phosphopolysaccharide also produced IL-1 alpha at a concentration of 50 micrograms/ml. This study showed for the first time that phosphopolysaccharide derived from a dairy lactic acid bacterium can induce IFN-gamma and IL-1 alpha production in macrophages. These findings strongly suggest that the phosphopolysaccharide is a type of 'biological response modifier' and the fermented dairy foods containing Lactococcus lactis ssp. cremoris can be designated as a physiologically functional food.
- Subjects :
- Lipopolysaccharides
Male
medicine.medical_treatment
Biology
Microbiology
chemistry.chemical_compound
Interferon-gamma
Mice
Interferon
medicine
Animals
Interferon gamma
RNA, Messenger
Cells, Cultured
Lactococcus lactis subsp cremoris
Macrophages
Lactococcus lactis
food and beverages
General Medicine
biology.organism_classification
Streptococcaceae
Lactic acid
Mice, Inbred C57BL
Cytokine
chemistry
Fermentation
Dairy Products
Spleen
Food Science
medicine.drug
Interleukin-1
Subjects
Details
- ISSN :
- 01681605
- Volume :
- 31
- Issue :
- 1-3
- Database :
- OpenAIRE
- Journal :
- International journal of food microbiology
- Accession number :
- edsair.doi.dedup.....19bdff4697f8843af5945cabd9ad9487